Methods in Molecular Biology

JAK-STAT Signalling

Methods and Protocols

Editors: Nicholson, Sandra E., Nicola, Nicos A. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

The receptor-associated JAK protein kinases and their substrates, the STAT transcriptional activators, transmit signals following cytokine and growth factor binding to receptors expressed on the cell surface, to result in specific transcriptional and cellular responses. Over the last two decades, the field has progressed from identification of the individual components through to an understanding of the activation and deactivation mechanisms, and the complex structural detail of the proteins involved. We now know that these pathways are important in many biological processes, including growth and development, hematopoiesis, and the innate and adaptive immune response. JAK-STAT Signalling: Methods and Protocols provides detailed methodologies for examining many aspects of the pathway. Divided into four sections, topics include JAK and STAT specific approaches, the negative regulators of the pathway (SOCS proteins), and the production and crystallization of JAK and STAT proteins, among others. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, JAK-STAT Signalling: Methods and Protocols will be of use not only to those working in the area but also to new investigators who are led to delve into the complexities of JAK and STAT responses.

Table of contents (21 chapters)

  • Analysis of Janus Tyrosine Kinase Phosphorylation and Activation

    Ross, Jeremy A. (et al.)

    Pages 3-20

  • Co-immunoprecipitation Protocol to Investigate Cytokine Receptor-Associated Proteins, e.g., Janus Kinases or Other Associated Signaling Proteins

    Haan, Claude (et al.)

    Pages 21-38

  • In Vitro JAK Kinase Activity and Inhibition Assays

    Babon, Jeffrey J. (et al.)

    Pages 39-55

  • Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Resonance

    Babon, Jeffrey J.

    Pages 57-67

  • Determination of Protein Turnover Rates in the JAK/STAT Pathway Using a Radioactive Pulse-Chase Approach

    Dittrich, Anna (et al.)

    Pages 69-80

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-242-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-241-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
JAK-STAT Signalling
Book Subtitle
Methods and Protocols
Editors
  • Sandra E. Nicholson
  • Nicos A. Nicola
Series Title
Methods in Molecular Biology
Series Volume
967
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science + Business Media New York
eBook ISBN
978-1-62703-242-1
DOI
10.1007/978-1-62703-242-1
Hardcover ISBN
978-1-62703-241-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 320
Topics