Methods in Molecular Biology

Antigen Processing

Methods and Protocols

Editors: van Endert, Peter (Ed.)

  • Details comprehensive methods to study antigen presentation by classical and non-classical MHC proteins
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-218-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-217-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Antigen processing is a biological process that prepares antigens for the presentation to special cells in the immune system called T lymphocytes. In Antigen Processing: Methods and Protocols, expert researchers in the field provide a comprehensive set of protocols for studying presentation of antigens produced in the standard processing pathways for MHC class I and class II molecules. The chapters follow chronology of intracellular processing events, ending with recognition of peptide-MHC complexes at the cell surface by T lymphocytes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Antigen Processing: Methods and Protocols is designed for beginners and experts interested in studying antigen processing.

Reviews

From the reviews:

“The book is divided into 42 chapters, each split into a short abstract, an introduction providing the relevant bibliographic overview, a detailed list of materials used in the protocol, a step-by-step description of the procedure, and a final section with notes. … Van Endert achieves what he set out to do – to provide an in-depth bench-side companion for antigen processing beginners and experts … . The book comes highly recommended.” (Clemens Hermann, Immunology News, February, 2014)

“The book is designed to be a benchside companion for researchers interested in studying antigen processing. It is intended for both beginners and experts studying antigen presentation.” (Omer Iqbal, Doody’s Book Reviews, August, 2013)

Table of contents (42 chapters)

  • Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes

    Tenzer, Stefan (et al.)

    Pages 1-14

  • Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry

    Textoris-Taube, Kathrin (et al.)

    Pages 15-29

  • Using Protease Inhibitors in Antigen Presentation Assays

    Basler, Michael (et al.)

    Pages 31-39

  • Kinetic Studies of Cytoplasmic Antigen Processing and Production of MHC Class I Ligands

    Stargardt, Anita (et al.)

    Pages 41-51

  • Assaying Peptide Translocation by the Peptide Transporter TAP

    Jongsma, Marlieke L. M. (et al.)

    Pages 53-65

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-218-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-217-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Antigen Processing
Book Subtitle
Methods and Protocols
Editors
  • Peter van Endert
Series Title
Methods in Molecular Biology
Series Volume
960
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-218-6
DOI
10.1007/978-1-62703-218-6
Hardcover ISBN
978-1-62703-217-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 589
Topics