Methods in Molecular Biology

Mammalian Oocyte Regulation

Methods and Protocols

Editors: Homer, Hayden A. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-191-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-62703-190-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-4939-5940-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Mammalian oocytes occupy a critical nexus in reproduction as they not only contribute half the genetic makeup of the embryo but also provide virtually all of the cytoplasmic building blocks required for sustaining embryogenesis. The journey that transforms a primordial germ cell into a mature oocyte (or egg) capable of fertilisation and embryonic development is of unrivalled complexity. The end-product of this remarkable developmental process is the “mature” egg, now replete with macromolecules and organelles, and poised to support embryonic development if fertilisation should occur. Given the importance of the oocyte for embryonic development, it is of no surprise that the oocyte holds centre stage in fertility clinics and greatly influences the outcome of assisted reproductive treatments (ARTs). Mammalian Oocyte Regulation: Methods and Protocols provides a highly diverse compendium of detailed methodologies - primarily focusing on the murine model but also including chapters on human oocytes - for enabling researchers to interrogate every aspect of mammalian oocyte development including recombination, meiotic maturation, oocyte substrate uptake, chromosomal segregation and fertilisation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Mammalian Oocyte Regulation: Methods and Protocols serves as an ideal guide to novice oocyte researchers, experts who wish to add another dimension to their established portfolio, as well as the wider research community.

Table of contents (21 chapters)

  • Studying Recombination in Mouse Oocytes

    Sun, Xianfei (et al.)

    Pages 1-18

  • Analysis of Recombinants in Female Mouse Meiosis

    Boer, Esther (et al.)

    Pages 19-45

  • Studying Meiosis-Specific Cohesins in Mouse Embryonic Oocytes

    Kim, Ji-hye (et al.)

    Pages 47-57

  • Immunohistochemical Approaches to the Study of Human Fetal Ovarian Development

    He, Jing (et al.)

    Pages 59-75

  • Protein Kinase Assays for Measuring MPF and MAPK Activities in Mouse and Rat Oocytes and Early Embryos

    Kubiak, Jacek Z.

    Pages 77-89

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-191-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-62703-190-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA
  • ISBN 978-1-4939-5940-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Mammalian Oocyte Regulation
Book Subtitle
Methods and Protocols
Editors
  • Hayden A. Homer
Series Title
Methods in Molecular Biology
Series Volume
957
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-191-2
DOI
10.1007/978-1-62703-191-2
Hardcover ISBN
978-1-62703-190-5
Softcover ISBN
978-1-4939-5940-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 311
Topics