Methods in Molecular Biology

Electron Crystallography of Soluble and Membrane Proteins

Methods and Protocols

Editors: Schmidt-Krey, Ingeborg, Cheng, Yifan (Eds.)

  • Covers the entire range of techniques used in electron crystallography, from protein sample preparation to modeling of conformational changes
  • Features detailed protocols suitable for labs with minimal equipment and subject familiarity and other techniques for the most state-of-the-art facilities
  • Includes key tips and expert implementation advice to ensure successful results
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-176-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-175-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The basic principle of electron crystallography is to calculate a 3D density map by combining the amplitudes obtained from electron diffraction patterns with the experimental phases calculated from images of two-dimensional crystals of membrane or soluble proteins. This technology is very well developed and has produced a number of atomic models of membrane proteins in a lipid environment.  Focused on comprehensive experimental protocols, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols covers the entire range of techniques used in electron crystallography, including protein sample preparation, 2D crystallization, and screening in negative stain over electron cryo-microscopy (cryo-EM) and data processing, as well as modeling of conformational changes. Additional chapters provide perspective on past, present, and future challenges as well as complementary methods.  Written for the popular Methods in Molecular Biology™ series, the work contains the kind of detailed descriptions and implementation advice necessary to ensure successful results.

 

Comprehensive and cutting-edge, Electron Crystallography of Soluble and Membrane Proteins: Methods and Protocols serves laboratories new to the methods as well as state-of-the-art facilities pursuing this exciting area of protein science.

Reviews

From the reviews:

“This book contains chapters covering all of the key elements of electron crystallography. … This is definitely a valuable book to have in every laboratory that is actively working in the area of electron crystallography because it provides a great deal of information in one convenient place. It would also be useful for anyone who just wants to get a general understanding about this branch of electron microscopy and structural biology, even without intending to use crystals in their own work.” (Robert Glaeser, Microscopy and Microanalysis, Vol. 19 (3), June, 2013)

Table of contents (30 chapters)

  • Introduction to Electron Crystallography

    Kühlbrandt, Werner

    Pages 1-16

  • Practical Aspects in Expression and Purification of Membrane Proteins for Structural Analysis

    Vinothkumar, Kutti R. (et al.)

    Pages 17-30

  • Two-Dimensional Crystallization of Membrane Proteins by Reconstitution Through Dialysis

    Johnson, Matthew C. (et al.)

    Pages 31-58

  • Monolayer Two-Dimensional Crystallization of Membrane Proteins

    Lebeau, Luc (et al.)

    Pages 59-71

  • Screening for Two-Dimensional Crystals by Transmission Electron Microscopy of Negatively Stained Samples

    Dreaden, Tina M. (et al.)

    Pages 73-101

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-176-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-175-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Electron Crystallography of Soluble and Membrane Proteins
Book Subtitle
Methods and Protocols
Editors
  • Ingeborg Schmidt-Krey
  • Yifan Cheng
Series Title
Methods in Molecular Biology
Series Volume
955
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-176-9
DOI
10.1007/978-1-62703-176-9
Hardcover ISBN
978-1-62703-175-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 586
Topics