Methods in Molecular Biology

Peroxisome Proliferator-Activated Receptors (PPARs)

Methods and Protocols

Editors: Badr, Mostafa Z., Youssef, Jihan A. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-155-4
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Hardcover $169.00
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About this book

All three peroxisome proliferator-activated receptor (PPAR) subtypes share a high degree of structural homology but differ in function, tissue distribution and ligand specificity. PPARs play critical roles as regulators of numerous physiological as well as pathophysiological pathways, and efforts are currently underway to fully characterize their functioning and to develop safer and more effective PPAR modulators to treat a myriad of diseases and conditions. In Peroxisome proliferator-Activated Receptors: Methods and Protocols, renowned experts in the PPAR arena provide detailed protocols for investigating these receptors. Chapters contain methods ranging from the cloning of receptors to their knockdown, to protocols exploring posttranslational modifications of PPARs and coactivators, as well as receptor subcellular localization. Also assembled are methods to evaluate the involvement of these receptors in behavior functions, an emerging facet in PPAR research. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

With its well-honed methodologies, Peroxisome proliferator-Activated Receptors: Methods and Protocols,  will be a useful resource for all seeking to advance their knowledge of this field.

Table of contents (25 chapters)

  • PPARs: History and Advances

    Youssef, Jihan (et al.)

    Pages 1-6

  • PPAR-Alpha Cloning, Expression, and Characterization

    Ngo, Suong N. T. (et al.)

    Pages 7-34

  • PPARGC1A and PPARG Genotyping: Beginner’s Guide to Genotyping with Unlabeled Probes

    Habalová, Viera (et al.)

    Pages 35-55

  • Generation of an Inducible, Cardiomyocyte-Specific Transgenic Mouse Model with PPAR β/δ Overexpression

    Kim, Teayoun (et al.)

    Pages 57-65

  • Specific Knockdown of PPARδ Gene in Colon Cancer Cells by Lentivirus-Mediated RNA Interfering

    Yang, Lie (et al.)

    Pages 67-86

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-155-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-154-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Peroxisome Proliferator-Activated Receptors (PPARs)
Book Subtitle
Methods and Protocols
Editors
  • Mostafa Z. Badr
  • Jihan A. Youssef
Series Title
Methods in Molecular Biology
Series Volume
952
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-62703-155-4
DOI
10.1007/978-1-62703-155-4
Hardcover ISBN
978-1-62703-154-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 343
Topics