Methods in Molecular Biology

Mass Spectrometry of Glycoproteins

Methods and Protocols

Editors: Kohler, Jennifer J., Patrie, Steven M. (Eds.)

  • Resource for those who work at the interface of glycobiology and mass spectrometry
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-62703-146-2
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  • Immediate eBook download after purchase
Hardcover $169.00
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About this book

 Glycosylation is the most abundant post-translational modification of proteins.  Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics.  These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol  is  an essential resource for those who work at the interface of glycobiology and mass spectrometry.

Table of contents (22 chapters)

  • Introduction to Glycosylation and Mass Spectrometry

    Patrie, Steven M. (et al.)

    Pages 1-17

  • Tandem Lectin Weak Affinity Chromatography for Glycoprotein Enrichment

    Ma, Zhi Yuan (et al.)

    Pages 21-31

  • CSC Technology: Selective Labeling of Glycoproteins by Mild Oxidation to Phenotype Cells

    Hofmann, Andreas (et al.)

    Pages 33-43

  • Use of Boronic Acid Nanoparticles in Glycoprotein Enrichment

    Xu, Yawei (et al.)

    Pages 45-55

  • Incorporation of Unnatural Sugars for the Identification of Glycoproteins

    Zaro, Balyn W. (et al.)

    Pages 57-67

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-146-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-145-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Mass Spectrometry of Glycoproteins
Book Subtitle
Methods and Protocols
Editors
  • Jennifer J. Kohler
  • Steven M. Patrie
Series Title
Methods in Molecular Biology
Series Volume
951
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-146-2
DOI
10.1007/978-1-62703-146-2
Hardcover ISBN
978-1-62703-145-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 339
Topics