Methods in Molecular Biology

Nanotechnology for Nucleic Acid Delivery

Methods and Protocols

Editors: Ogris, Manfred, Oupicky, David (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-140-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-62703-139-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Nanotechnology and nucleic acid based therapies are two emerging fields in science whose combination has the potential to improve quality of life for patients suffering from various diseases that can so far only be treated in an unsatisfactory way. Nucleic acids offer the potential for highly selective treatment of such diseases or the highly specific modulation of gene expression with RNA interference. A key issue for successful nucleic acid therapies is the availability of a suitable delivery system. Here, the field of nanotechnology offers a multitude of possibilities to develop nanosized delivery vectors tailor-made for various local and systemic approaches. In Nanotechnology for Nucleic Acid Delivery: Methods and Protocols, experts in the field cover the area of nanoparticulate delivery of nucleic acids in terms of biosafety, particle synthesis as well as its application in cell culture. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Nanotechnology for Nucleic Acid Delivery: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies.

Table of contents (19 chapters)

  • Toxicological Aspects for Nanomaterial in Humans

    Dusinska, Maria (et al.)

    Pages 1-12

  • Lactate Dehydrogenase Assay for Assessment of Polycation Cytotoxicity

    Parhamifar, Ladan (et al.)

    Pages 13-22

  • Combined Fluorimetric Caspase 3/7 Assay and Bradford Protein Determination for Assessment of Polycation-Mediated Cytotoxicity

    Larsen, Anna K. (et al.)

    Pages 23-33

  • Anti-PEG IgM Production via a PEGylated Nano-Carrier System for Nucleic Acid Delivery

    Ichihara, Masako (et al.)

    Pages 35-47

  • Near-Infrared Optical Imaging of Nucleic Acid Nanocarriers In Vivo

    Rome, Claire (et al.)

    Pages 49-65

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-140-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.00
price for USA
  • ISBN 978-1-62703-139-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Nanotechnology for Nucleic Acid Delivery
Book Subtitle
Methods and Protocols
Editors
  • Manfred Ogris
  • David Oupicky
Series Title
Methods in Molecular Biology
Series Volume
948
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-140-0
DOI
10.1007/978-1-62703-140-0
Hardcover ISBN
978-1-62703-139-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 286
Topics