Methods in Molecular Biology

Basic Cell Culture Protocols

Editors: Helgason, Cheryl D., Miller, Cindy L. (Eds.)

  • Contains numerous new chapters that explore the most current areas of investigation as well as updated chapters from the previous edition
  • Includes clear and concise protocols, ready for the laboratory
  • Features expert tips and key implementation advice to ensure successful results
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-128-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-127-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems.  Written in the highly successful Methods in Molecular Biology™, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Table of contents (33 chapters)

  • Detection of Mycoplasma Contaminations

    Uphoff, Cord C. (et al.)

    Pages 1-13

  • Eradication of Mycoplasma Contaminations

    Uphoff, Cord C. (et al.)

    Pages 15-26

  • STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination

    Dirks, Wilhelm G. (et al.)

    Pages 27-38

  • Classical and Molecular Cytogenetic Analysis

    MacLeod, Roderick A. F. (et al.)

    Pages 39-60

  • Fluorescent In Situ Hybridization of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle

    Cannizzaro, Linda A.

    Pages 61-83

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-62703-128-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-62703-127-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Basic Cell Culture Protocols
Editors
  • Cheryl D. Helgason
  • Cindy L. Miller
Series Title
Methods in Molecular Biology
Series Volume
946
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: Mehul Book Sales, Mumbai, India
eBook ISBN
978-1-62703-128-8
DOI
10.1007/978-1-62703-128-8
Hardcover ISBN
978-1-62703-127-1
Series ISSN
1064-3745
Edition Number
4
Number of Pages
XIV, 550
Topics