Methods in Molecular Biology

Epithelial Cell Culture Protocols

Editors: Randell, Scott H., Fulcher, M. Leslie (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts.
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-125-7
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  • Immediate eBook download after purchase
Hardcover $169.00
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  • ISBN 978-1-62703-124-0
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About this book

Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.

Table of contents (26 chapters)

  • Progress Towards Drosophila Epithelial Cell Culture

    Simcox, Amanda

    Pages 1-11

  • Culture of Choroid Plexus Epithelial Cells and In Vitro Model of Blood–CSF Barrier

    Monnot, Andrew D. (et al.)

    Pages 13-29

  • Methods for Culture of Human Corneal and Conjunctival Epithelia

    Spurr-Michaud, Sandra J. (et al.)

    Pages 31-43

  • The Culture and Maintenance of Functional Retinal Pigment Epithelial Monolayers from Adult Human Eye

    Blenkinsop, Timothy A. (et al.)

    Pages 45-65

  • Oral and Pharyngeal Epithelial Keratinocyte Culture

    Leelahavanichkul, Kantima (et al.)

    Pages 67-79

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-125-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-124-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Epithelial Cell Culture Protocols
Editors
  • Scott H. Randell
  • M. Leslie Fulcher
Series Title
Methods in Molecular Biology
Series Volume
945
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-125-7
DOI
10.1007/978-1-62703-125-7
Hardcover ISBN
978-1-62703-124-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XII, 469
Topics