Methods in Molecular Biology

siRNA Design

Methods and Protocols

Editors: Taxman, Debra J. (Ed.)

  • Assists researchers, educators, clinicians, and biotech scientists in harnessing the power of RNAi
  • Features easy to follow protocols and techniques for siRNA design
  • Includes expert tips and implementation advice to ensure successful results
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-119-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-118-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi.  siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance.  The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume.  Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results.

 

Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.

Reviews

From the reviews:

“This book edited by D.J. Taxman is a valuable collection of 20 chapters covering aspects from values for parameters used in tools for designing siRNAs to screening methods for evaluating the efficiency of the designed siRNA. … Through style and scientific information the book is aimed at researchers in the RNAi field, but its accessible delivery of contents makes it a good starting point for younger researchers in this topic.” (Irina Ioana Mohorianu, zbMATH, Vol. 1276, 2014)

Table of contents (20 chapters)

  • What Parameters to Consider and Which Software Tools to Use for Target Selection and Molecular Design of Small Interfering RNAs

    Matveeva, Olga

    Pages 1-16

  • Methods for Selecting Effective siRNA Target Sequences Using a Variety of Statistical and Analytical Techniques

    Takasaki, Shigeru

    Pages 17-55

  • Designing Functional siRNA with Reduced Off-Target Effects

    Naito, Yuki (et al.)

    Pages 57-68

  • Design and Screening of siRNAs Against Highly Structured RNA Targets

    Nasheri, Neda (et al.)

    Pages 69-86

  • Engineering Small Interfering RNAs by Strategic Chemical Modification

    Bramsen, Jesper B. (et al.)

    Pages 87-109

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-119-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-118-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
siRNA Design
Book Subtitle
Methods and Protocols
Editors
  • Debra J. Taxman
Series Title
Methods in Molecular Biology
Series Volume
942
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-119-6
DOI
10.1007/978-1-62703-119-6
Hardcover ISBN
978-1-62703-118-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 392
Topics