Methods in Molecular Biology

Recombinant and In Vitro RNA Synthesis

Methods and Protocols

Editors: Conn, Graeme L. (Ed.)

  • Details a collection of approaches that guide the reader from the start of a new RNA project to the final sample
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-113-4
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Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-112-7
  • Free shipping for individuals worldwide
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Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5830-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.

Table of contents (19 chapters)

  • Purification of RNA Expressed In Vivo Inserted in a tRNA Scaffold

    Ponchon, Luc (et al.)

    Pages 1-8

  • Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold

    Ponchon, Luc (et al.)

    Pages 9-18

  • Preparation of Long Templates for RNA In Vitro Transcription by Recursive PCR

    Bowman, Jessica C. (et al.)

    Pages 19-41

  • General Protocols for Preparation of Plasmid DNA Template, RNA In Vitro Transcription, and RNA Purification by Denaturing PAGE

    Linpinsel, Jo L. (et al.)

    Pages 43-58

  • Preparation of Short RNA by In Vitro Transcription

    Lu, Cheng (et al.)

    Pages 59-68

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-62703-113-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-62703-112-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5830-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Recombinant and In Vitro RNA Synthesis
Book Subtitle
Methods and Protocols
Editors
  • Graeme L. Conn
Series Title
Methods in Molecular Biology
Series Volume
941
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-113-4
DOI
10.1007/978-1-62703-113-4
Hardcover ISBN
978-1-62703-112-7
Softcover ISBN
978-1-4939-5830-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 274
Topics