Methods in Molecular Biology

Biolistic DNA Delivery

Methods and Protocols

Editors: Sudowe, Stephan, Reske-Kunz, Angelika B. (Eds.)

  • Covers biolistic gene transfer into plants, nematodes, or in vitro and in vivo mammalian cells
  • Includes lab-ready protocols with clear step-by-step direction from leading researchers in the field
  • Features key tips and implementation advice to guarantee successful results
see more benefits

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-110-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-109-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun.  Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

All-inclusive and cutting-edge,  Biolistic DNA Delivery: Methods and Protocols brings together the knowledge and the experience of leading experts in the field of gene transfer in order to serve all researchers who wish to further our abilities in this vital field.

Table of contents (29 chapters)

  • Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun

    Gao, Caixia (et al.)

    Pages 3-16

  • Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery

    Ueki, Shoko (et al.)

    Pages 17-26

  • Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues

    Sujatha, Mulpuri (et al.)

    Pages 27-44

  • Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-vacuum Gene Gun (HandyGun)

    Vuorinen, Anssi L. (et al.)

    Pages 45-51

  • HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies

    Gaba, Victor (et al.)

    Pages 53-62

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-110-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-109-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Biolistic DNA Delivery
Book Subtitle
Methods and Protocols
Editors
  • Stephan Sudowe
  • Angelika B. Reske-Kunz
Series Title
Methods in Molecular Biology
Series Volume
940
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-110-3
DOI
10.1007/978-1-62703-110-3
Hardcover ISBN
978-1-62703-109-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 415
Topics