Methods in Molecular Biology

Calcium Signaling Protocols

Editors: Lambert, David G., Rainbow, Richard D. (Eds.)

  • Combines brand new protocols with heavily revised and improved methodologies
  • Features lab-ready procedures with step-by-step instruction
  • Includes expert tips and key implementation advice to ensure successful results
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-086-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-085-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.  With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes.  Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+.

Table of contents (21 chapters)

  • Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations

    Simpson, Alec W. M.

    Pages 3-36

  • Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2

    Patel, Anish (et al.)

    Pages 37-47

  • Confocal Microscopy: Theory and Applications for Cellular Signaling

    Tovey, Stephen C. (et al.)

    Pages 51-93

  • Ratiometric Ca2+ Measurements Using the FlexStation®Scanning Fluorometer

    Marshall, Ian C. B. (et al.)

    Pages 95-101

  • Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®)

    Marshall, Ian C. B. (et al.)

    Pages 103-109

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-086-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-085-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Calcium Signaling Protocols
Editors
  • David G. Lambert
  • Richard D. Rainbow
Series Title
Methods in Molecular Biology
Series Volume
937
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-086-1
DOI
10.1007/978-1-62703-086-1
Hardcover ISBN
978-1-62703-085-4
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XI, 360
Topics