Methods in Molecular Biology

MicroRNA Protocols

Editors: Ying, Shao-Yao (Ed.)

  • Fully updates and expands upon the successful miRNA methodologies of the first edition
  • Features potential applications in cosmetics, wound healing, and prostate cancer
  • Includes key tips and implementation advice to ensure successful results
see more benefits

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-62703-083-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-1-62703-082-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Extensive studies have been conducted on the identification, biogenesis, and processing of microRNA (miRNA) as well as research on the exact mechanism by which miRNAs bring about translational silencing of their targets. In addition, numerous publications point to an important role of miRNAs in development, reprogramming, epigenetics, pathogenesis of cancer, oncogenes and tumor suppressors, biomarkers of various disease onset, and regulation of adipogenesis and obesity; yet many questions still remain.  In MicroRNA Protocols, Second Edition, experts in the field provide up-to-date coverage of this diverse area of study.  The specific chapters of this edition are related to the analysis of miRNA, targets and expression profiling, various methods to determine its regulation of gene expression, the preparation and isolation of miRNAs in specific tissues, its detection in the saliva, and potential application in cosmetics, wound healing, and prostate cancer.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and known pitfalls.

 

Fully updated and easy to use, MicroRNA Protocols, Second Edition aims to stimulate readers to explore diverse ways to understand the mechanisms in which miRNAs facilitate the molecular aspects of not only biomedical research but also a wide range of other research fields.

Table of contents (27 chapters)

  • The MicroRNA

    Ying, Shao-Yao (et al.)

    Pages 1-19

  • Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer

    Starega-Roslan, Julia (et al.)

    Pages 21-34

  • A User-Friendly Computational Workflow for the Analysis of MicroRNA Deep Sequencing Data

    Majer, Anna (et al.)

    Pages 35-45

  • The RNA Gene Information: Retroelement-MicroRNA Entangling as the RNA Quantum Code

    Fujii, Yoichi Robertus

    Pages 47-67

  • Setting Up an Intronic miRNA Database

    Hinske, Ludwig Christian (et al.)

    Pages 69-76

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-62703-083-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-1-62703-082-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
MicroRNA Protocols
Editors
  • Shao-Yao Ying
Series Title
Methods in Molecular Biology
Series Volume
936
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-083-0
DOI
10.1007/978-1-62703-083-0
Hardcover ISBN
978-1-62703-082-3
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 375
Topics