Methods in Molecular Biology

Spermatogenesis

Methods and Protocols

Editors: Carrell, Douglas, Aston, Kenneth I. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-038-0
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  • Immediate eBook download after purchase
Hardcover $169.00
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About this book

Deficiencies in sperm function are usually the result of spermatogenic defects. Spermatogenesis is a biologically complex and essential process during which spermatogonia undergo meiotic recombination, reduction of the genome to a haploid state, and extensive cellular modifications that result in a motile cell capable of traversing the female reproductive tract, withstanding various potential assaults to viability, and finally successfully fertilizing a mature oocyte to give rise to an embryo. Defects in any step of spermatogenesis or spermatogenesis can lead to male infertility, a disease that affects approximately 5-7% of the population. Spermiogenesis and Spermatogenesis: Methods and Protocols details protocols used in the study of spermatogenesis, clinical analytical protocols, and basic techniques used in clinical andrology laboratories, such as obtaining accurate results for a sperm count, and advanced procedures, such as genome-wide genetic study tools and evaluation of nuclear proteins. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Spermiogenesis and Spermatogenesis: Methods and Protocols is unique in its breadth, and will be a useful reference for clinicians and researchers alike.

Table of contents (45 chapters)

  • Methods for Sperm Concentration Determination

    Björndahl, Lars

    Pages 3-12

  • Methods of Sperm Vitality Assessment

    Moskovtsev, Sergey I. (et al.)

    Pages 13-19

  • The Hypo-osmotic Swelling Test for Evaluation of Sperm Membrane Integrity

    Ramu, Sivakumar (et al.)

    Pages 21-25

  • Sperm Morphology Classification: A Rational Method for Schemes Adopted by the World Health Organization

    Rothmann, Susan A. (et al.)

    Pages 27-37

  • Sperm Morphology Assessment Using Strict (Tygerberg) Criteria

    Menkveld, Roelof

    Pages 39-50

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-62703-038-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-62703-037-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Spermatogenesis
Book Subtitle
Methods and Protocols
Editors
  • Douglas Carrell
  • Kenneth I. Aston
Series Title
Methods in Molecular Biology
Series Volume
927
Copyright
2013
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-62703-038-0
DOI
10.1007/978-1-62703-038-0
Hardcover ISBN
978-1-62703-037-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 554
Topics