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Genomic Imprinting

Methods and Protocols

  • Book
  • © 2012

Overview

  • Surveys cutting-edge technologies applied in the field
  • Features detailed and practical laboratory protocols
  • Includes expert tips on troubleshooting and avoiding pitfalls
  • Includes supplementary material: sn.pub/extras
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 925)

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Table of contents (19 protocols)

  1. Parent-of-Origin Effects

  2. Identifying Imprinted Genes

  3. Identifying the Regulatory Features of Imprinted Domains

  4. Epigenetics of Imprinted Regions

  5. Analysis of Imprinted Expression

Keywords

About this book

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting.  This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in  unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.

Editors and Affiliations

  • School of Medicine, Fels Institute, Temple University, Philadelphia, USA

    Nora Engel

Bibliographic Information

  • Book Title: Genomic Imprinting

  • Book Subtitle: Methods and Protocols

  • Editors: Nora Engel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-011-3

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-62703-010-6Published: 21 August 2012

  • Softcover ISBN: 978-1-4939-5966-2Published: 23 August 2016

  • eBook ISBN: 978-1-62703-011-3Published: 21 August 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 297

  • Topics: Human Genetics

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