Methods in Molecular Biology

Antibody Engineering

Methods and Protocols, Second Edition

Editors: Chames, Patrick (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $159.00
price for USA (gross)
  • ISBN 978-1-61779-974-7
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  • Immediate eBook download after purchase
Hardcover $209.00
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About this book

More than ever, antibodies are being recognized as a major drug modality in a variety of diseases, including cancer, autoimmune diseases, infectious diseases, or even neurodegenerative disorders. Over 30 therapeutic antibodies have been approved and novel molecules are entering clinical trials at an average rate of 50 per year and that is predicted to continue well into the future. Notwithstanding the many achievements already made in the field, there is still a lot of room for improvements for these molecules in terms of activity, and a plethora of approaches have been attempted to optimize these molecules. Antibody Engineering: Methods and Protocols, Second Edition was compiled to give complete and easy access to a variety of antibody engineering techniques, starting from the creation of antibody repertoires and efficient ways to select binders from these repertoires, to their production in various hosts, their detailed characterization using various well established techniques, and to the modification and optimization of these lead molecules in terms of binding activity, specificity, size, shape, and more. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Antibody Engineering: Methods and Protocols, Second Edition serves as an invaluable resource for both experts and those new to the field, and most of all as a source of inspiration for the creation of the antibodies of tomorrow.

Table of contents (40 chapters)

  • Use of IMGT® Databases and Tools for Antibody Engineering and Humanization

    Lefranc, Marie-Paule (et al.)

    Pages 3-37

  • Computer-Assisted Modeling of Antibody Variable Domains

    Ramos, Oscar H. P.

    Pages 39-55

  • Cloning Single-Chain Antibody Fragments (ScFv) from Hyrbidoma Cells

    Toleikis, Lars (et al.)

    Pages 59-71

  • Human In-Cell scFv Library from Infiltrating B Cell

    Peraldi-Roux, Sylvie

    Pages 73-83

  • Construction of Human Naive Antibody Gene Libraries

    Hust, Michael (et al.)

    Pages 85-107

Buy this book

eBook $159.00
price for USA (gross)
  • ISBN 978-1-61779-974-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $209.00
price for USA
  • ISBN 978-1-61779-973-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Antibody Engineering
Book Subtitle
Methods and Protocols, Second Edition
Editors
  • Patrick Chames
Series Title
Methods in Molecular Biology
Series Volume
907
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-974-7
DOI
10.1007/978-1-61779-974-7
Hardcover ISBN
978-1-61779-973-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 734
Topics