Methods in Molecular Biology

Single Domain Antibodies

Methods and Protocols

Editors: Saerens, Dirk, Muyldermans, Serge (Eds.)

  • Features methods of selection, expression, improvement, and library construction of single domain antibodies
  • Includes step-by-step instruction that has been tested in labs around the world
  • Provides key tips and implementation advice that is essential for success
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eBook $139.00
price for USA (gross)
  • ISBN 978-1-61779-968-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-61779-967-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications; however, the bulky size of mAbs, costly production, and cumbersome engineering hampered regularly their streamlined development in some applications.  In Single Domain Antibodies: Methods and Protocols, expert researchers examine single variable domain antibody fragments, referred to as VH, VL, VHH or VNAR. These fragments are the smallest intact antigen-binding fragments that can be produced recombinantly at low cost.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Table of contents (36 chapters)

  • From Whole Monoclonal Antibodies to Single Domain Antibodies: Think Small

    Teillaud, Jean-Luc

    Pages 3-13

  • Introduction to Heavy Chain Antibodies and Derived Nanobodies

    Vincke, Cécile (et al.)

    Pages 15-26

  • Overview and Discovery of IgNARs and Generation of VNARs

    Nuttall, Stewart D.

    Pages 27-36

  • Creation of the Large and Highly Functional Synthetic Repertoire of Human VH and Vκ Domain Antibodies

    Ignatovich, Olga (et al.)

    Pages 39-63

  • Preparation of a Naïve Library of Camelid Single Domain Antibodies

    Olichon, Aurelien (et al.)

    Pages 65-78

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-61779-968-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-61779-967-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Single Domain Antibodies
Book Subtitle
Methods and Protocols
Editors
  • Dirk Saerens
  • Serge Muyldermans
Series Title
Methods in Molecular Biology
Series Volume
911
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-968-6
DOI
10.1007/978-1-61779-968-6
Hardcover ISBN
978-1-61779-967-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 580
Number of Illustrations and Tables
54 b/w illustrations, 35 illustrations in colour
Topics