Methods in Molecular Biology

Quantitative Methods in Proteomics

Editors: Marcus, Katrin (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-885-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-884-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Protein modifications and changes made to them, as well as the quantities of expressed proteins, can define the various functional stages of the cell. Accordingly, perturbations can lead to various diseases and disorders. As a result, it has become paramount to be able to detect and monitor post-translational modifications and to measure the abundance of proteins within the cell with extreme sensitivity. While protein identification is an almost routine requirement nowadays, reliable techniques for quantifying unmodified proteins (including those that escape detection under standard conditions, such as protein isoforms and membrane proteins) is not routine. Quantitative Methods in Proteomics gives a detailed survey of topics and methods on the principles underlying modern protein analysis, from statistical issues when planning proteomics experiments, to gel-based and mass spectrometry-based applications. The quantification of post-translational modifications is also addressed, followed by the “hot” topics of software and data analysis, as well as various overview chapters which provide a comprehensive overview of existing methods in quantitative proteomics. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Quantitative Methods in Proteomics serves as a comprehensive and competent overview of the important and still growing field of quantitative proteomics.

Table of contents (32 chapters)

  • Important Issues in Planning a Proteomics Experiment: Statistical Considerations of Quantitative Proteomic Data

    Podwojski, Katharina (et al.)

    Pages 3-21

  • The Whereabouts of 2D Gels in Quantitative Proteomics

    Rabilloud, Thierry

    Pages 25-35

  • Proteome Analysis with Classical 2D-PAGE

    May, Caroline (et al.)

    Pages 37-46

  • Fast and Sensitive Coomassie Staining in Quantitative Proteomics

    Dyballa, Nadine (et al.)

    Pages 47-59

  • Silver Staining of 2D Electrophoresis Gels

    Rabilloud, Thierry

    Pages 61-73

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-885-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-884-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Quantitative Methods in Proteomics
Editors
  • Katrin Marcus
Series Title
Methods in Molecular Biology
Series Volume
893
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-885-6
DOI
10.1007/978-1-61779-885-6
Hardcover ISBN
978-1-61779-884-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 539
Number of Illustrations and Tables
86 b/w illustrations, 11 illustrations in colour
Topics