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Gene Synthesis

Methods and Protocols

  • Book
  • © 2012

Overview

Editors:
  1. Jean Peccoud

    1. Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, USA

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  • Defines gene synthesis and determines what part of the process labs can do and what parts can be contracted to a specialized service provider
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 852)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Assembly of Oligonucleotides in Synthons

    1. Front Matter

      Pages 1-1
      Download chapter PDF

  3. Assembly of oligonucleotides in synthons

    1. Building Block Synthesis Using the Polymerase Chain Assembly Method

      • Julie A. Marchand, Jean Peccoud
      Pages 3-10

    2. Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments

      • Daniel G. Gibson
      Pages 11-21

    3. TopDown Real-Time Gene Synthesis

      • Mo Chao Huang, Wai Chye Cheong, Hongye Ye, Mo-Huang Li
      Pages 23-34

    4. De Novo DNA Synthesis Using Single-Molecule PCR

      • Tuval Ben Yehezkel, Gregory Linshiz, Ehud Shapiro
      Pages 35-47

  4. Synthon Assembly

    1. Front Matter

      Pages 49-49
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    2. SLIC: A Method for Sequence- and Ligation-Independent Cloning

      • Mamie Z. Li, Stephen J. Elledge
      Pages 51-59

    3. Assembly of Standardized DNA Parts Using BioBrick Ends in E. coli

      • Olivia Ho-Shing, Kin H. Lau, William Vernon, Todd T. Eckdahl, A. Malcolm Campbell
      Pages 61-76

    4. Assembling DNA Fragments by USER Fusion

      • Narayana Annaluru, Héloïse Muller, Sivaprakash Ramalingam, Karthikeyan Kandavelou, Viktoriya London, Sarah M. Richardson et al.
      Pages 77-95

    5. Fusion PCR via Novel Overlap Sequences

      • Kamonchai Cha-aim, Hisashi Hoshida, Tomoaki Fukunaga, Rinji Akada
      Pages 97-110

    6. Using Recombineering to Generate Point Mutations: The Oligonucleotide-Based “Hit and Fix” Method

      • Suhwan Chang, Stacey Stauffer, Shyam K. Sharan
      Pages 111-120

    7. Using Recombineering to Generate Point Mutations:galK-Based Positive–Negative Selection Method

      • Kajal Biswas, Stacey Stauffer, Shyam K. Sharan
      Pages 121-131

    8. Assembling Large DNA Segments in Yeast

      • Héloïse Muller, Narayana Annaluru, Joy Wu Schwerzmann, Sarah M. Richardson, Jessica S. Dymond, Eric M. Cooper et al.
      Pages 133-150

    9. Recursive Construction of Perfect DNA Molecules and Libraries from Imperfect Oligonucleotides

      • Gregory Linshiz, Tuval Ben Yehezkel, Ehud Shapiro
      Pages 151-163

    10. Cloning Whole Bacterial Genomes in Yeast

      • Gwynedd A. Benders
      Pages 165-180

    11. Production of Infectious Poliovirus from Synthetic Viral Genomes

      • Jeronimo Cello, Steffen Mueller
      Pages 181-193

  5. Software for Gene Synthesis

    1. Front Matter

      Pages 195-195
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  6. Software for gene synthesis

    1. In Silico Design of Functional DNA Constructs

      • Alan Villalobos, Mark Welch, Jeremy Minshull
      Pages 197-213

    2. Using DNAWorks in Designing Oligonucleotides for PCR-Based Gene Synthesis

      • David Hoover
      Pages 215-223
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Keywords

About this book

The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.

Editors and Affiliations

  • Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, USA

    Jean Peccoud

Bibliographic Information

  • Book Title: Gene Synthesis

  • Book Subtitle: Methods and Protocols

  • Editors: Jean Peccoud

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-564-0

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Sceince+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-563-3Published: 10 February 2012

  • Softcover ISBN: 978-1-4939-6200-6Published: 23 August 2016

  • eBook ISBN: 978-1-61779-564-0Published: 11 February 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 297

  • Number of Illustrations: 35 b/w illustrations, 42 illustrations in colour

  • Topics: Human Genetics, Genetic Engineering

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