Methods in Molecular Biology

Gene Synthesis

Methods and Protocols

Editors: Peccoud, Jean (Ed.)

  • Defines gene syntheseis and determines what part of the process labs can do and what parts should be contracted to a specialized service provider
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-564-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-563-3
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  • Usually dispatched within 3 to 5 business days.
About this book

The de novo fabrication of custom DNA molecules is a transformative technology that significantly affects the biotechnology industry. Basic genetic engineering techniques for manipulating DNA in vitro opened an incredible field of opportunity in the life sciences. In, Gene Synthesis: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to fabricate DNA . These include methods and techniques for the assembly of oligonucleotide, cloning of synthons into larger fragments, protocols and software applications, and educational and biosecurity impacts of gene synthesis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Gene Synthese: Methods and Protocols aids scientists in understanding all the different stages of a complex gene synthesis process, while refining their understanding of gene synthesis and determine what part of the process they can or should do in their laboratory and what parts should be contracted to a specialized service provider.

Table of contents (21 chapters)

  • Building Block Synthesis Using the Polymerase Chain Assembly Method

    Marchand, Julie A. (et al.)

    Pages 3-10

  • Oligonucleotide Assembly in Yeast to Produce Synthetic DNA Fragments

    Gibson, Daniel G.

    Pages 11-21

  • TopDown Real-Time Gene Synthesis

    Huang, Mo Chao (et al.)

    Pages 23-34

  • De Novo DNA Synthesis Using Single-Molecule PCR

    Yehezkel, Tuval Ben (et al.)

    Pages 35-47

  • SLIC: A Method for Sequence- and Ligation-Independent Cloning

    Li, Mamie Z. (et al.)

    Pages 51-59

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-564-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-563-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Gene Synthesis
Book Subtitle
Methods and Protocols
Editors
  • Jean Peccoud
Series Title
Methods in Molecular Biology
Series Volume
852
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Sceince+Business Media, LLC
eBook ISBN
978-1-61779-564-0
DOI
10.1007/978-1-61779-564-0
Hardcover ISBN
978-1-61779-563-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 297
Number of Illustrations and Tables
35 b/w illustrations, 42 illustrations in colour
Topics