Methods in Molecular Biology

Host-Fungus Interactions

Methods and Protocols

Editors: Brand, Alexandra C., MacCallum, Donna M. (Eds.)

  • Examines the culture and genetic manipulation of primary fungal pathogens as well as interactions in model systems
  • Includes detailed, tested methods that are ready for the lab
  • Features key tips and implementation advice necessary to achieve successful results
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Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-539-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-538-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Microbiologists, medical mycologists, immunologists, and biochemists are increasingly working together to focus on the processes involved in the progression and treatment of fungal disease.  Host-Fungus Interactions: Methods and Protocols is designed for research scientists who are involved in this work and interested in undertaking new or comparative studies of interactions between the mammalian host and clinically important fungal pathogens. Aiming to combine approaches for reverse genetics in pathogenic fungi with methods for their application in in vitro and in vivo models of disease, the book includes methods for the culture and genetic manipulation of the primary fungal pathogens and the opportunistic pathogens, as well as methods for investigating host-fungus interactions in model systems.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and practical, Host-Fungus Interactions: Methods and Protocols describes available molecular methods and fungal infection models in great detail in order to encourage researchers to try new approaches to investigating host-fungus interactions with added levels of confidence.

Reviews

From the book reviews:

“The book ‘Host-fungus Interactions’ assembles methods and protocols for studying gene disruption, modulation of gene expression, host and fungal responses to infection and several model systems to analyze the host-fungus interactions. The book comprises of 8 parts with 42 chapters and an index at the end of the book. … A useful tool for medical microbiologists, immunologists, geneticists, biotechnologists and students studying pharmacology and pathogenicity testing.” (Melvina D’souza and Kevin D. Hyde, Fungal Diversity, December, 2014)

Table of contents (42 chapters)

  • Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy

    Sasse, Christoph (et al.)

    Pages 3-17

  • Mini-blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

    Ganguly, Shantanu (et al.)

    Pages 19-39

  • Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans

    Lenardon, Megan D. (et al.)

    Pages 41-49

  • Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatum

    Zemska, Olga (et al.)

    Pages 51-66

  • Targeted Gene Disruption in Cryptococcus neoformans Using Double-Joint PCR with Split Dominant Selectable Markers

    Kim, Min Su (et al.)

    Pages 67-84

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-539-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-538-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Host-Fungus Interactions
Book Subtitle
Methods and Protocols
Editors
  • Alexandra C. Brand
  • Donna M. MacCallum
Series Title
Methods in Molecular Biology
Series Volume
845
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-539-8
DOI
10.1007/978-1-61779-539-8
Hardcover ISBN
978-1-61779-538-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 597
Number of Illustrations and Tables
67 b/w illustrations, 37 illustrations in colour
Topics