Methods in Molecular Biology

Neurotrophic Factors

Methods and Protocols

Editors: Skaper, Stephen D (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.

Reviews

From the reviews:

“It provides a foundation for neurobiological research into the effects of neurotrophic factors and other chemicals on the nervous system. … The audience is broad, including practicing and in training neuroscientists, neurobiologists, and basic neuroscience researchers in human and veterinary medicine, as well as academic neurologists, neurosurgeons, and pharmacists. The methods can be incorporated into basic research laboratories and into medical, biology, and veterinary classes.” (Ramsis Farid Ghaly, Doody’s Book Reviews, July, 2012)

Table of contents (33 chapters)

  • The Neurotrophin Family of Neurotrophic Factors: An Overview

    Skaper, Stephen D.

    Pages 1-12

  • Neuronal Growth-Promoting and Inhibitory Cues in Neuroprotection and Neuroregeneration

    Skaper, Stephen D.

    Pages 13-22

  • Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents

    Facci, Laura (et al.)

    Pages 23-37

  • Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum

    Zusso, Morena (et al.)

    Pages 39-47

  • Culture of Rodent Cortical and Hippocampal Neurons

    Facci, Laura (et al.)

    Pages 49-56

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-536-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-535-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Neurotrophic Factors
Book Subtitle
Methods and Protocols
Editors
  • Stephen D Skaper
Series Title
Methods in Molecular Biology
Series Volume
846
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-536-7
DOI
10.1007/978-1-61779-536-7
Hardcover ISBN
978-1-61779-535-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 421
Topics