Methods in Molecular Biology

Ancient DNA

Methods and Protocols

Editors: Shapiro, Beth, Hofreiter, Michael (Eds.)

  • Closes the gap between appropriate methodological approaches and retrieval and anylsis of ancient DNA
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-516-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-515-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Research into ancient DNA began more than 25 years ago with the publication of short mitochondrial DNA sequence fragments from the quagga, an extinct relative of the zebra. Ancient DNA research really gained momentum following the invention of PCR, which allowed millions of copies to be made of the few remaining DNA molecules preserved in fossils and museum specimens.  In Ancient DNA: Methods and Protocols expert researchers in the field describe many of the protocols that are now commonly used to study ancient DNA. These include instructions for setting up an ancient DNA laboratory, extraction protocols for a wide range of different substrates, details of laboratory techniques including PCR and NGS library preparation, and suggestions for appropriate analytical approaches to make sense of the sequences obtained. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Ancient DNA: Methods and Protocols seeks to aid scientists in the further study of ancient DNA and the methodological approaches in ancient research.

Table of contents (24 chapters)

  • Setting Up an Ancient DNA Laboratory

    Fulton, Tara L.

    Pages 1-11

  • A Phenol–Chloroform Protocol for Extracting DNA from Ancient Samples

    Barnett, Ross (et al.)

    Pages 13-19

  • DNA Extraction of Ancient Animal Hard Tissue Samples via Adsorption to Silica Particles

    Rohland, Nadin

    Pages 21-28

  • Case Study: Recovery of Ancient Nuclear DNA from Toe Pads of the Extinct Passenger Pigeon

    Fulton, Tara L. (et al.)

    Pages 29-35

  • Extraction of DNA from Paleofeces

    Kuch, Melanie (et al.)

    Pages 37-42

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-516-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-515-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Ancient DNA
Book Subtitle
Methods and Protocols
Editors
  • Beth Shapiro
  • Michael Hofreiter
Series Title
Methods in Molecular Biology
Series Volume
840
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-516-9
DOI
10.1007/978-1-61779-516-9
Hardcover ISBN
978-1-61779-515-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 247
Number of Illustrations and Tables
14 b/w illustrations, 12 illustrations in colour
Topics