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  • © 2012

Planar Cell Polarity

Methods and Protocols

Editors:

  • Gathers together timely protocols on its vital subject area
  • Features lab-ready methods that have been proven successful
  • Includes key tips and expert implementation advice
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 839)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
  2. Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Locations

    • Daryl J. V. David, Melanie A. McGill, R. F. Andrew McKinley, Tony J. C. Harris
    Pages 1-17
  3. Analysis of Cell Shape and Polarity During Zebrafish Gastrulation

    • Douglas C. Weiser, David Kimelman
    Pages 53-68
  4. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling

    • Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, Ira O. Daar
    Pages 91-104
  5. Gene Loss-of-Function and Live Imaging in Chick Embryos

    • Anne C. Rios, Christophe Marcelle, Olivier Serralbo
    Pages 105-117
  6. Activation and Function of Small GTPases Rho, Rac, and Cdc42 During Gastrulation

    • Courtney Mezzacappa, Yuko Komiya, Raymond Habas
    Pages 119-131
  7. Convergent Extension Analysis in Mouse Whole Embryo Culture

    • Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D. E. Greene, Andrew J. Copp
    Pages 133-146
  8. Analysis of PCP Defects in Mammalian Eye Lens

    • Yuki Sugiyama, John W. McAvoy
    Pages 147-156
  9. Examining Planar Cell Polarity in the Mammalian Cochlea

    • Helen May-Simera, Matthew W. Kelley
    Pages 157-171
  10. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration

    • Kristi LaMonica, Laura Grabel
    Pages 187-200
  11. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells

    • Mitsuharu Endo, Michiru Nishita, Yasuhiro Minami
    Pages 201-214
  12. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling

    • Shinji Matsumoto, Akira Kikuchi
    Pages 215-227
  13. The Embryonic Mouse Gut Tube as a Model for Analysis of Epithelial Polarity

    • Makoto Matsuyama, Akihiko Shimono
    Pages 229-237
  14. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models

    • Daniel J. Jagger, Andrew Forge
    Pages 239-248

About this book

Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states.  In Planar Cell Polarity: Methods and Protocols, expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world.  As a volume in the highly successful Methods in Molecular Biology™ series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study.

Editors and Affiliations

  • Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Planar Cell Polarity

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-510-7

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-509-1Published: 05 January 2012

  • Softcover ISBN: 978-1-4939-5906-8Published: 30 April 2017

  • eBook ISBN: 978-1-61779-510-7Published: 06 January 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 262

  • Number of Illustrations: 32 b/w illustrations, 27 illustrations in colour

  • Topics: Cell Biology, Cell Physiology

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access