Methods in Molecular Biology

Mitochondrial Disorders

Biochemical and Molecular Analysis

Editors: Wong, Lee-Jun C. (Ed.)

  • Presents cutting-edge molecular and biochemical methods of analysis
  • Features detailed, practical laboratory protocols
  • Includes tips from the experts on how to best successfully complete the methods
see more benefits

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-504-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-503-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Due to the complexity of the mitochondrial system and the myriad genes involved in their function, the clinical manifestations of mitochondrial diseases are remarkably variable and heterogeneous. The laboratory and clinical methodologies used to evaluate dysfunction may vary widely, and thusly a systematic presentation of the numerous protocols that are applied to the assessment of these clinically and genetically heterogeneous disorders has proven to be essential.  Mitochondrial Disorders: Biochemical and Molecular Analysis strives to fill this need with a collection of key protocols provided by leading experts in the field.  Beginning with overviews of complexity of mitochondrial and nuclear genome disorders, the book continues with a section devoted to current biochemical protocols and a part focused on the DNA-based approaches used to identify molecular defects.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Detailed and authoritative, Mitochondrial Disorders: Biochemical and Molecular Analysis serves as a vital guide to researchers seeking to understand this incredibly complicated type of breakdown in cellular biology.

Table of contents (23 chapters)

  • Mitochondrial DNA Mutations: An Overview of Clinical and Molecular Aspects

    Craigen, William J.

    Pages 3-15

  • Nuclear Gene Defects in Mitochondrial Disorders

    Scaglia, Fernando

    Pages 17-34

  • Diagnostic Challenges of Mitochondrial Disorders: Complexities of Two Genomes

    Graham, Brett H.

    Pages 35-46

  • Biochemical Analyses of the Electron Transport Chain Complexes by Spectrophotometry

    Frazier, Ann E. (et al.)

    Pages 49-62

  • Measurement of Mitochondrial Oxygen Consumption Using a Clark Electrode

    Li, Zhihong (et al.)

    Pages 63-72

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-504-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-503-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Mitochondrial Disorders
Book Subtitle
Biochemical and Molecular Analysis
Editors
  • Lee-Jun C. Wong
Series Title
Methods in Molecular Biology
Series Volume
837
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-504-6
DOI
10.1007/978-1-61779-504-6
Hardcover ISBN
978-1-61779-503-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 353
Topics