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  • © 2012

Microbial Metabolic Engineering

Methods and Protocols

Editors:

  • Strategies and techniques of metabolic engineering within the scope of microbial applications mainly focused on bacteria and yeasts
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 834)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xii
  2. Screening for Cellulases with Industrial Value and Their Use in Biomass Conversion

    • Julia Jüergensen, Nele Ilmberger, Wolfgang R. Streit
    Pages 1-16
  3. Reversal of NAD(P)H Cofactor Dependence by Protein Engineering

    • Sabine Bastian, Frances H. Arnold
    Pages 17-31
  4. Quantifying Plasmid Copy Number to Investigate Plasmid Dosage Effects Associated with Directed Protein Evolution

    • Samuel Million-Weaver, David L. Alexander, Jennifer M. Allen, Manel Camps
    Pages 33-48
  5. Recombination-Based DNA Assembly and Mutagenesis Methods for Metabolic Engineering

    • Xiquan Liang, Lansha Peng, Billyana Tsvetanova, Ke Li, Jian-Ping Yang, Tony Ho et al.
    Pages 93-109
  6. Use of Proteomic Tools in Microbial Engineering for Biofuel Production

    • Shaoming Mao, Kaizhi Jia, Yanping Zhang, Yin Li
    Pages 137-151
  7. Use FACS Sorting in Metabolic Engineering of Escherichia coli for Increased Peptide Production

    • Qiong Cheng, Kristin Ruebling-Jass, Jianzhong Zhang, Qi Chen, Kevin M. Croker
    Pages 177-196
  8. Using Flux Balance Analysis to Guide Microbial Metabolic Engineering

    • Kathleen A. Curran, Nathan C. Crook, Hal S. Alper
    Pages 197-216
  9. Preparation and Evaluation of Lignocellulosic Biomass Hydrolysates for Growth by Ethanologenic Yeasts

    • Ying Zha, Ronald Slomp, Johan van Groenestijn, Peter J. Punt
    Pages 245-259
  10. Metabolic Engineering for Acetate Control in Large Scale Fermentation

    • Yong Tao, Qiong Cheng, Alexander D. Kopatsis
    Pages 283-303

About this book

Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study metabolic engineering. These include methods and techniques to engineer genes and pathways, use of modern biotechnology tools in microbial metabolic engineering, and examples of metabolic engineering for real world applications such as whole cell biosensors and acetate control in large scale fermentation. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Microbial Metabolic Engineering: Methods and Protocols seeks to provide researchers with an overview of key topics on microbial metabolic engineering.

Editors and Affiliations

  • Central Research & Development, E.I. Du Pont de Nemours and Company, Wilmington, USA

    Qiong Cheng

Bibliographic Information

  • Book Title: Microbial Metabolic Engineering

  • Book Subtitle: Methods and Protocols

  • Editors: Qiong Cheng

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-483-4

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2012

  • Hardcover ISBN: 978-1-61779-482-7Published: 06 December 2011

  • Softcover ISBN: 978-1-4939-5909-9Published: 23 August 2016

  • eBook ISBN: 978-1-61779-483-4Published: 07 December 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 319

  • Topics: Microbiology, Biochemical Engineering

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access