Methods in Molecular Biology

Astrocytes

Methods and Protocols

Editors: Milner, Richard (Ed.)

  • Provides a selection of the key cellular, molecular,and biochemical techniques that are used in studying the many varied functions of this fascinating cell
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-61779-452-0
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  • Immediate eBook download after purchase
Hardcover $189.00
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  • ISBN 978-1-61779-451-3
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About this book

Ever since their discovery more than 150 years ago, astrocytes have proved to be something of an enigma.  Only in the last 10-15 years has it become clear that astrocytes are multifunctional versatile cells that play key roles in a multitude of diverse processes in the CNS, including generation of neural stem cells, synaptogenesis, and regulation of the blood-brain barrier and neurovascular unit. Astrocytes: Methods and Protocols provides scientists with a comprehensive guide to many techniques used for astrocyte cell culture, as well as more specialized approaches for studying astrocyte functions, both in vitro and in vivo. Key cellular, molecular and biochemical techniques are used to study the many and varied functions of this fascinating cell.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Table of contents (37 chapters)

  • Astrocytes: Multitalented Stars of the Central Nervous System

    Ransom, Bruce R. (et al.)

    Pages 3-7

  • Neurogenic Astrocytes and Their Glycoconjugates: Not Just “Glue” Anymore

    Steindler, Dennis A.

    Pages 9-22

  • Heterogeneity of Astrocytic Form and Function

    Oberheim, Nancy Ann (et al.)

    Pages 23-45

  • Preparation of Mixed Glial Cultures from Postnatal Rat Brain

    Vellis, Jean (et al.)

    Pages 49-59

  • Generation of Primary Astrocyte Cultures Devoid of Contaminating Microglia

    Uliasz, Tracy F. (et al.)

    Pages 61-79

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-452-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-451-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Astrocytes
Book Subtitle
Methods and Protocols
Editors
  • Richard Milner
Series Title
Methods in Molecular Biology
Series Volume
814
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-452-0
DOI
10.1007/978-1-61779-452-0
Hardcover ISBN
978-1-61779-451-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 575
Topics