Methods in Molecular Biology

Germline Development

Methods and Protocols

Editors: Chan, Wai-Yee, Blomberg, Le Ann (Eds.)

  • Features techniques specific to both male and female germlines
  • Provides easily accessible, step-by-step protocols that are ready for the lab
  • Includes expert tips and implementation advice to ensure successful results
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-436-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-435-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert researchers provide a wide-ranging collection of techniques that can be applied to the study of both the male and female germline.  As the techniques used for isolating and studying male and female germ cells are slightly dissimilar, the different technologies are grouped under two sections in this volume, namely, male germline and female germline.  This collection brings the field of germline developmental studies together such that readers can find, in a single volume, techniques that allow the isolation of gonads with germ cells at different stages of development, the study of the genomics and epigenomics of germ cells, and an examination of the role of single genes in the developmental potential and function of germ cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and the avoidance of known pitfalls. 

 

Comprehensive and cutting-edge, Germline Development: Methods and Protocols serves as a comprehensive text to individuals, particularly researchers in reproductive and developmental biology, who are interested in studying cells of the germ lineage.

Table of contents (18 chapters)

  • Isolation of Fetal Gonads from Embryos of Timed-Pregnant Mice for Morphological and Molecular Studies

    Li, Yunmin (et al.)

    Pages 3-16

  • Neonatal Testicular Gonocytes Isolation and Processing for Immunocytochemical Analysis

    Manku, Gurpreet (et al.)

    Pages 17-29

  • Isolation of Undifferentiated and Early Differentiating Type A Spermatogonia from Pou5f1-GFP Reporter Mice

    Garcia, Thomas (et al.)

    Pages 31-44

  • Isolation of Human Male Germ-Line Stem Cells Using Enzymatic Digestion and Magnetic-Activated Cell Sorting

    He, Zuping (et al.)

    Pages 45-57

  • Isolation and Purification of Murine Male Germ Cells

    Boucheron, Catherine (et al.)

    Pages 59-66

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-436-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-435-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Germline Development
Book Subtitle
Methods and Protocols
Editors
  • Wai-Yee Chan
  • Le Ann Blomberg
Series Title
Methods in Molecular Biology
Series Volume
825
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-436-0
DOI
10.1007/978-1-61779-436-0
Hardcover ISBN
978-1-61779-435-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 256
Topics