Methods in Molecular Biology

Recombinant Gene Expression

Editors: Lorence, Argelia (Ed.)

  • Contains new protocols and topics not yet covered
  • Provides step-by-step detail essentaial for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-433-9
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  • Immediate eBook download after purchase
Hardcover $189.00
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  • ISBN 978-1-61779-432-2
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About this book

Studies related to recombinant gene expression have brought new advance such as  the emergence of the “omics” technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.

Table of contents (35 chapters)

  • Using Folding Promoting Agents in Recombinant Protein Production: A Review

    Fahnert, Beatrix

    Pages 3-36

  • Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry

    Savary, Brett J. (et al.)

    Pages 37-50

  • A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression

    Cruz Muñoz-Centeno, María (et al.)

    Pages 51-64

  • Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins

    Dolan, Maureen C. (et al.)

    Pages 65-105

  • Heat-Shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli

    Kyratsous, Christos A. (et al.)

    Pages 109-129

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-433-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-432-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Recombinant Gene Expression
Editors
  • Argelia Lorence
Series Title
Methods in Molecular Biology
Series Volume
824
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
Distribution Rights
Distribution rights for India: CBS Publishers, New Delhi, India
eBook ISBN
978-1-61779-433-9
DOI
10.1007/978-1-61779-433-9
Hardcover ISBN
978-1-61779-432-2
Series ISSN
1064-3745
Edition Number
3
Number of Pages
XVI, 649
Number of Illustrations and Tables
105 b/w illustrations
Topics