Methods in Molecular Biology

SELDI-TOF Mass Spectrometry

Methods and Protocols

Editors: Clarke, Charlotte H., McCarthy, Diane L. Bankert (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-418-6
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Hardcover $139.00
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  • ISBN 978-1-61779-417-9
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About this book

SELDI is distinct from other TOF-MS technologies in that it couples features of chromatography and mass spectrometry, facilitating analyte enrichment and sample cleanup on an array surface. In the growing field of proteomics, SELDI technology has been widely used for biomarker discovery and characterization in diverse applications including diagnostics, drug development, and basic research. SELDI-based biomarker studies can typically be divided into four phases: discovery, validation, purification and identification, and assay development. SELDI-TOF Mass Spectrometry: Methods and Protocols provides an overview of the current applications of SELDI-TOF MS (surface enhanced laser desorption/ionization time-of-flight mass spectrometry), with an emphasis on study and experimental design, data analysis and interpretation, and assay development. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, SELDI-TOF Mass Spectrometry: Methods and Protocols will provide information on optimizing study design, experimental protocols, and data analysis and interpretation to yield robust biomarkers and biomarker assays, using examples from different disease areas.

Table of contents (16 chapters)

  • Optimized Conditions for a Quantitative SELDI TOF MS Protein Assay

    Lomas, Lee (et al.)

    Pages 1-10

  • Solid-Phase Fractionation Strategies Applied to Proteomics Investigations

    Guerrier, Luc (et al.)

    Pages 11-33

  • Data Processing and Analysis Using ProteinChip® Data Manager Software

    Dalmasso, Enrique A. (et al.)

    Pages 35-48

  • Purification and Identification of Candidate Biomarkers Discovered Using SELDI-TOF MS

    Bulman, Amanda L. (et al.)

    Pages 49-66

  • Biomarker Discovery in Serum/Plasma Using Surface Enhanced Laser Desorption Ionization Time of Flight (SELDI–TOF) Mass Spectrometry

    Ndao, Momar

    Pages 67-79

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-418-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-417-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
SELDI-TOF Mass Spectrometry
Book Subtitle
Methods and Protocols
Editors
  • Charlotte H. Clarke
  • Diane L. Bankert McCarthy
Series Title
Methods in Molecular Biology
Series Volume
818
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-418-6
DOI
10.1007/978-1-61779-418-6
Hardcover ISBN
978-1-61779-417-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 239
Number of Illustrations and Tables
50 b/w illustrations, 4 illustrations in colour
Topics