Methods in Molecular Biology

Bone Research Protocols

Editors: Helfrich, Miep H., Ralston, Stuart H. (Eds.)

  • Contains a catalogue of protocols to assist researchers in the pursuit of mechanistic studies
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-61779-415-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-61779-414-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Studies over the past decade have continued to bring tremendous advances to our understanding of bone biology. New pathways have been discovered and expanded our knowledge of the ways in which genes and gene products affect bone cells and thereby bone mass and bone strength. In Bone Research Protocols, Second Edition, expert researchers in the field detail many methods commonly used to study bone biology. Focusing mainly on in vitro methods, this volume gives techniques for isolation, culture and functional analysis of all bone cell types and details a range of imaging methods, including light and ultrastructural microscopy and live cell imaging. Some important in vivo techniques are included, such as analysis of bone resorption and imaging using X rays, fluorescent or luminescent techniques. Methods for study of proteins and nucleic acid are included and methods for analysis of bone composition, measurement of bone strength, and response to mechanical stimulation are described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Bone Research Protocols, Second Edition seeks to aid scientists in the bone field to establish new techniques in their laboratories.

Reviews

From the reviews of the second edition:

“This volume presents detailed modern experimental methods to study bone biology. For bone researchers.” (Pediatric Endocrinology Reviews (PER), Vol. 9 (3), March, 2012)

Table of contents (38 chapters)

  • Primary Human Osteoblast Cultures

    Dillon, Jane P. (et al.)

    Pages 3-18

  • Osteoblast Isolation from Murine Calvaria and Long Bones

    Bakker, Astrid D. (et al.)

    Pages 19-29

  • Rat Osteoblast Cultures

    Orriss, Isabel R. (et al.)

    Pages 31-41

  • Isolation of Primary Avian Osteocytes

    Semeins, Cor M. (et al.)

    Pages 43-53

  • Isolation of Mouse Osteocytes Using Cell Fractionation for Gene Expression Analysis

    Halleux, Christine (et al.)

    Pages 55-66

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-61779-415-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-61779-414-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Bone Research Protocols
Editors
  • Miep H. Helfrich
  • Stuart H. Ralston
Series Title
Methods in Molecular Biology
Series Volume
816
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-415-5
DOI
10.1007/978-1-61779-415-5
Hardcover ISBN
978-1-61779-414-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 607
Topics