Methods in Molecular Biology

Transcriptional Regulation

Methods and Protocols

Editors: Vancura, Ales (Ed.)

  • Presents detailed protocols developed using mammalian, yeast, and Drosophila cells and tissues, which can easily be applied across species and cell types
  • Features reliable methods tested in laboratories around the world
  • Includes expert tips and implementation advice to ensure successful results
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-376-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
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  • Immediate eBook download after purchase
Hardcover $189.00
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  • ISBN 978-1-61779-375-2
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About this book

Through many recent remarkable developments, perhaps the most significant advancements in the study of transcriptional regulation are the development of genome-wide approaches for measuring gene expression, exemplified by gene chips (chip), and chromatin immunoprecipitation assays (ChIP) for measuring in vivo protein-DNA interactions at any genomic loci. Transcriptional Regulation: Methods and Protocols takes this progress and builds upon it with a collection of key protocols used in expert laboratories around the world.  Divided into four convenient sections, this detailed volume explores promoter elements, transcription factors, and preinitiation complex (PIC) assembly, chromatin structure, chromatin modifying complexes, and RNA synthesis and regulation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and accessible, Transcriptional Regulation: Methods and Protocols equally serves senior researchers and scientists experienced in transcriptional regulation as well as graduate students and scientists who wish to study transcriptional regulation for the first time.

Table of contents (39 chapters)

  • Genome-Wide In Vivo Cross-linking of Sequence-Specific Transcription Factors

    Li, Xiao-Yong (et al.)

    Pages 3-26

  • Characterization of Complex Regulatory Networks and Identification of Promoter Regulatory Elements in Yeast: “In Silico” and “Wet-Lab” Approaches

    Mira, Nuno P. (et al.)

    Pages 27-48

  • Electrophoretic Mobility Shift Assay Analysis of NFκB Transcriptional Regulation by Nuclear IκBα

    Juvekar, Ashish (et al.)

    Pages 49-62

  • Probing Endogenous RNA Polymerase II Pre-initiation Complexes by Electrophoretic Mobility Shift Assay

    Wilhelm, Emmanuelle (et al.)

    Pages 63-74

  • Elucidating Protein: DNA Complex by Oligonucleotide DNA Affinity Purification

    Yang, Teddy T. C. (et al.)

    Pages 75-84

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-376-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-375-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Transcriptional Regulation
Book Subtitle
Methods and Protocols
Editors
  • Ales Vancura
Series Title
Methods in Molecular Biology
Series Volume
809
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-376-9
DOI
10.1007/978-1-61779-376-9
Hardcover ISBN
978-1-61779-375-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 627
Number of Illustrations and Tables
104 b/w illustrations
Topics