Methods in Molecular Biology

Protein Expression in Mammalian Cells

Methods and Protocols

Editors: Hartley, James L (Ed.)

  • Explores techniques to express proteins in mammalian cells in order to relate the research most closely to human biology
  • Features step-by-step methods and protocols, ready for the lab
  • Includes expert tips and implementation advice to ensure successful results
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-352-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-351-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease.  In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

 

Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.

Reviews

From the reviews:

“This book is really a great help for all those that wish to enter the field and I am sure even for those who are already involved with recombinant protein production in mammalian cells. … the book is intended as well for basic researchers aimed to study native or recombinant proteins to highlight … protein misfolding or the role of proteins in cancer and in general is intended for all those scientists aiming to contribute to solve the many mysteries still connoting the protein world.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)


Table of contents (16 chapters)

  • Why Proteins in Mammalian Cells?

    Hartley, James L.

    Pages 1-12

  • Large-Scale Transfection of Mammalian Cells

    Baldi, Lucia (et al.)

    Pages 13-26

  • Selection of High Expressing Mammalian Cells by Surface Display of Reporters

    DeMaria, Christine T.

    Pages 27-39

  • Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles

    Jardin, Barbara Ann (et al.)

    Pages 41-63

  • Transfection of Difficult-to-Transfect Primary Mammalian Cells

    Gresch, Oliver (et al.)

    Pages 65-74

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-352-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-351-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Expression in Mammalian Cells
Book Subtitle
Methods and Protocols
Editors
  • James L Hartley
Series Title
Methods in Molecular Biology
Series Volume
801
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-352-3
DOI
10.1007/978-1-61779-352-3
Hardcover ISBN
978-1-61779-351-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 276
Topics