Methods in Molecular Biology

Allostery

Methods and Protocols

Editors: Fenton, Aron W. (Ed.)

  • Contains expert protocols, the common feature of which are allosteric systems, i.e. ligand-induced, through-protein changes
  • Draws together the study of protein structure/function and the study of allostery
  • Features key tips and implementation advice to ensure successful results
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-334-9
  • Digitally watermarked, DRM-free
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  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-333-2
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About this book

Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function.  In Allostery: Methods and Protocols, expert researchers in the field provide key techniques to investigate this biological phenomenon.  Focusing on heterotropic systems with some coverage of homotropic systems, this volume covers the monitoring of allosteric function, allosteric conformational changes, and allosteric changes in protein dynamics/sub-population distribution, as well as topics such as macromolecular and ligand engineering of allosteric functions and computational aids in the study of allostery. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Allostery: Methods and Protocols aids scientists in continuing to study ligand-induced, through-protein effects on protein function (ligand binding/catalysis), a phenomenon that is well recognized through the history of the life sciences and very poorly understood at the molecular level.

Table of contents (23 chapters)

  • Binding Techniques to Study the Allosteric Energy Cycle

    Kranz, James K. (et al.)

    Pages 3-17

  • Kinetic Trapping of a Key Hemoglobin Intermediate

    Holt, Jo M. (et al.)

    Pages 19-29

  • Allosteric Coupling Between Transition Metal-Binding Sites in Homooligomeric Metal Sensor Proteins

    Grossoehme, Nicholas E. (et al.)

    Pages 31-51

  • Studying the Allosteric Energy Cycle by Isothermal Titration Calorimetry

    Martinez-Julvez, Marta (et al.)

    Pages 53-70

  • Detecting “Silent” Allosteric Coupling

    Fisher, Harvey F.

    Pages 71-96

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-334-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-333-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Allostery
Book Subtitle
Methods and Protocols
Editors
  • Aron W. Fenton
Series Title
Methods in Molecular Biology
Series Volume
796
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-334-9
DOI
10.1007/978-1-61779-334-9
Hardcover ISBN
978-1-61779-333-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVIII, 439
Number of Illustrations and Tables
134 b/w illustrations
Topics