Methods in Molecular Biology

Unnatural Amino Acids

Methods and Protocols

Editors: Pollegioni, Loredano, Servi, Stefano (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
see more benefits

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-331-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-330-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of  unnatural amino acids in peptides with antimicrobial properties, genetic  incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.

Table of contents (27 chapters)

  • Preparation of Unnatural Amino Acids with Ammonia-Lyases and 2,3-Aminomutases

    Poppe, László (et al.)

    Pages 3-19

  • Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids

    D’Arrigo, Paola (et al.)

    Pages 21-35

  • Enzymatic Production of Enantiopure Amino Acids from Mono-substituted Hydantoin Substrates

    Matcher, Gwynneth F. (et al.)

    Pages 37-54

  • Preparation of Glutamate Analogues by Enzymatic Transamination

    Gefflaut, Thierry (et al.)

    Pages 55-72

  • Carbon–Carbon Bond-Forming Enzymes for the Synthesis of Non-natural Amino Acids

    Clapés, Pere (et al.)

    Pages 73-85

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-331-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-330-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Unnatural Amino Acids
Book Subtitle
Methods and Protocols
Editors
  • Loredano Pollegioni
  • Stefano Servi
Series Title
Methods in Molecular Biology
Series Volume
794
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-331-8
DOI
10.1007/978-1-61779-331-8
Hardcover ISBN
978-1-61779-330-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 409
Number of Illustrations and Tables
123 b/w illustrations
Topics