Methods in Molecular Biology

Handbook of ELISPOT

Methods and Protocols

Editors: Kalyuzhny, Alexander E. (Ed.)

  • Extends and expands upon the hands-on methodologies of the popular first edition
  • Features expert techniques ready for the laboratory
  • Includes key tips and implementation advice to ensure successful results
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-325-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-324-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research.  The second edition of Handbook of ELISPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition.  Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool.

Reviews

From the reviews of the second edition:

“In this second edition … the reader is introduced not only to the basic concept behind the technique but also to many different applications in common use. Importantly, the book serves as a reference guide with lots of trouble shooting and discussion of common problems. … This text would be key reading material for any laboratory planning to set up this technique. … this text is comprehensive and detailed with a good use of raw data and formula to make following the protocols simple.” (Hayley Evans, Immunology News, November, 2012)

Table of contents (19 chapters)

  • Unique Strengths of ELISPOT for T Cell Diagnostics

    Lehmann, Paul V. (et al.)

    Pages 3-23

  • The Impact of Harmonization on ELISPOT Assay Performance

    Janetzki, Sylvia (et al.)

    Pages 25-36

  • Equine ELISPOT Assay to Study Secretion of IFNγ and IL-4 from Peripheral Blood Mononuclear Cells

    Hagen, Jodi (et al.)

    Pages 39-45

  • Utilization of Feline ELISPOT for Mapping Vaccine Epitopes

    Abbott, Jeffrey R. (et al.)

    Pages 47-63

  • Analyzing Cellular Immunity to AAV in a Canine Model Using ELISPOT Assay

    Wang, Zejing (et al.)

    Pages 65-74

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-325-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-324-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Handbook of ELISPOT
Book Subtitle
Methods and Protocols
Editors
  • Alexander E. Kalyuzhny
Series Title
Methods in Molecular Biology
Series Volume
792
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-325-7
DOI
10.1007/978-1-61779-325-7
Hardcover ISBN
978-1-61779-324-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
X, 261
Number of Illustrations and Tables
46 b/w illustrations, 13 illustrations in colour
Topics