Methods in Molecular Biology

Epigenetics Protocols

Editors: Tollefsbol, Trygve (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-61779-316-5
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  • Immediate eBook download after purchase
Hardcover $169.00
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About this book

The field of epigenetics has played a major role at the forefront not only of molecular biology, but also of medical genetics and clinical medicine.  Few disciplines have experienced growth comparable to that which we have witnessed for epigenetics in the past decade. The goal of Epigenetics Protocols, Second Edition is to highlight select techniques that have been mainstays in the field as well as to cover methods that are especially relevant to extant discoveries in epigenetics.  This volume focuses on the two broad areas of epigenetics: DNA methylation and chromatin modifications, and also covers the complex topic of computational methods for epigenetic analyses which is essential to a complete understanding of the vast body of information that is being derived with the use of these newly-developed tools.  Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Epigenetics Protocols, Second Edition serves as an ideal guide to advanced students, basic scientists and clinical researchers as well as clinicians and biotechnology investigators who wish to continue exploring this exciting and progressive research field.

Table of contents (23 chapters)

  • Advances in Epigenetic Technology

    Tollefsbol, Trygve O.

    Pages 1-10

  • DNA Methylation Detection: Bisulfite Genomic Sequencing Analysis

    Li, Yuanyuan (et al.)

    Pages 11-21

  • Methylation-Specific PCR

    Ku, Ja-Lok (et al.)

    Pages 23-32

  • Analysing DNA Methylation Using Bisulphite Pyrosequencing

    Mikeska, Thomas (et al.)

    Pages 33-53

  • Closed-Tube PCR Methods for Locus-Specific DNA Methylation Analysis

    Candiloro, Ida L. M. (et al.)

    Pages 55-71

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-316-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-315-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Epigenetics Protocols
Editors
  • Trygve Tollefsbol
Series Title
Methods in Molecular Biology
Series Volume
791
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-316-5
DOI
10.1007/978-1-61779-316-5
Hardcover ISBN
978-1-61779-315-8
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XI, 332
Number of Illustrations and Tables
54 b/w illustrations
Topics