Methods in Molecular Biology

Cell Cycle Checkpoints

Methods and Protocols

Editors: Li, Willis X. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-61779-273-1
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  • Immediate eBook download after purchase
Hardcover $169.00
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About this book

Cell cycle checkpoints control the fidelity and orderly progression of eukaryotic cell division. By controlling the orderly progression of critical cell cycle events such as DNA replication and chromosome segregation and ensuring proper repair of damaged DNA, cell cycle checkpoints function to ensure genome integrity. Mechanisms of checkpoint controls are not only the research focus of investigators interested in mechanisms that regulate the cell cycle, but are also the interests of researchers studying cancer development as it is increasingly clear that loss of cell cycle checkpoints, which leads to genomic instability as a result, is a hallmark of tumorigenesis. Cell Cycle Checkpoints: Methods and Protocols provides detailed descriptions of methodologies currently employed by researchers in the field, including those commonly used in the mammalian, yeast, C. elegans, Drosophila, and Xenopus model systems. Each chapter describes a specific technique or protocol, such as a method to induce cell cycle checkpoints in a particular model system, to synchronize a population of cells to allow observations of cell cycle progression, to identify genes involved in checkpoint regulation, and to study particular protein components of cell cycle checkpoint pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Cell Cycle Checkpoints: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential field.

Table of contents (19 chapters)

  • Studying G2 DNA Damage Checkpoints Using the Fission Yeast Schizosaccharomyces pombe

    Willis, Nicholas (et al.)

    Pages 1-12

  • Studying S-Phase DNA Damage Checkpoints Using the Fission Yeast Schizosaccharomyces pombe

    Willis, Nicholas (et al.)

    Pages 13-21

  • Methods for Studying the G2 DNA Damage Checkpoint in Mammalian Cells

    Tapia-Alveal, Claudia (et al.)

    Pages 23-31

  • Evaluating Spindle Assembly Checkpoint Competence in Mouse Oocytes Using Immunoblotting

    Homer, Hayden

    Pages 33-45

  • Analysis of Changes in Protein Level and Subcellular Localization During Cell Cycle Progression Using the Budding Yeast Saccharomyces cerevisiae

    Wu, Xiaorong (et al.)

    Pages 47-57

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-273-1
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-272-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cell Cycle Checkpoints
Book Subtitle
Methods and Protocols
Editors
  • Willis X. Li
Series Title
Methods in Molecular Biology
Series Volume
782
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-273-1
DOI
10.1007/978-1-61779-273-1
Hardcover ISBN
978-1-61779-272-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 307
Number of Illustrations and Tables
68 b/w illustrations
Topics