Methods in Molecular Biology

Poly(ADP-ribose) Polymerase

Methods and Protocols

Editors: Tulin, Alexei V. (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-61779-270-0
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Hardcover $189.00
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About this book

Poly (ADP-ribose) Polymerases (PARPs) are abundant and ubiquitous proteins that regulate crucial processes of the cell cycle, DNA repair, genomic stability, and transcriptional regulation.  Being involved in basic cell functions, PARPs mediate rapid responses to such environmental factors as stress, infection, nutrition and hormonal signals. Whereas PARP inhibitors can suppress tumor growth and proliferation in certain breast, ovarian, and prostate cancers, understanding how PARP controls cellular functions is essential for the development of novel cancer treatments strategies. Divided into three convenient sections, Poly(ADP-Ribose) Polymerase: Methods and Protocols aims to explain how PARP proteins act within the normal development of an organism as well as in pathogenic conditions, seeks to advance the knowledge of developmental pathways regulation, and endeavors to facilitate the development of new therapeutic drugs and methods to target PARP-dependent processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Poly(ADP-Ribose) Polymerase: Methods and Protocols serves as an ideal guide to scientists who wish to continue exploring this exciting and progressive research field.

 

Table of contents (30 chapters)

  • Approaches to Detect PARP-1 Activation In Vivo, In Situ, and In Vitro

    Shah, Girish M. (et al.)

    Pages 3-34

  • Quantification of Poly(ADP-Ribose) In Vitro: Determination of the ADP-Ribose Chain Length and Branching Pattern

    Alvarez-Gonzalez, Rafael (et al.)

    Pages 35-46

  • Assay for Protein Modification by Poly-ADP-Ribose In Vitro

    Olabisi, Opeyemi A. (et al.)

    Pages 47-55

  • Identification of Distinct Amino Acids as ADP-Ribose Acceptor Sites by Mass Spectrometry

    Rosenthal, Florian (et al.)

    Pages 57-66

  • Noncovalent Protein Interaction with Poly(ADP-Ribose)

    Malanga, Maria (et al.)

    Pages 67-82

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-270-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-269-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Poly(ADP-ribose) Polymerase
Book Subtitle
Methods and Protocols
Editors
  • Alexei V. Tulin
Series Title
Methods in Molecular Biology
Series Volume
780
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-270-0
DOI
10.1007/978-1-61779-270-0
Hardcover ISBN
978-1-61779-269-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 521
Topics