Springer Protocols Handbooks

Human Embryonic and Induced Pluripotent Stem Cells

Lineage-Specific Differentiation Protocols

Editors: Ye, Kaiming, Jin, Sha (Eds.)

  • Serves to inspire the next generation of regenerative medicine and tissue engineering technologies
  • Features an extensive collection of protocols developed by leading scientists in the field
  • Contains practical laboratory guidance to promote successful results
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Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-267-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-266-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Because of the huge potential of human embryonic stem (hES) cells, especially the newly developed human induced pluripotent stem (hiPS) cells, in disease treatment and life quality improvement, enormous efforts have been made to develop new methodologies to translate lab discoveries in stem cell research into bed-side clinical technologies. In Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols, experts in the field present a comprehensive collection of protocols designed for labs around the world.  The topics covered in this detailed volume include techniques used for maintenance of hES and iPS cells in either small or large scale, techniques for directing hES and iPS cell lineage specification, techniques for enhancing the maturity of differentiated hES and iPS cells within three-dimensional scaffolds, techniques for reprogramming adult cells into iPS cells, techniques for generating patient-specific iPS cells, and techniques for translating hES and iPS cell research into new therapies.  Chapters include lab ready protocols with tips on troubleshooting and avoiding known pitfalls.

 

Wide-ranging and authoritative, Human Embryonic and Induced Pluripotent Stem Cells: Lineage-Specific Differentiation Protocols will be a tremendous aid for researchers and students who wish to explore these areas and transform their discoveries into the next generation of regenerative medicine and tissue engineering technologies.

Table of contents (34 chapters)

  • Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

    Hwang, Dong-Youn

    Pages 3-12

  • Growth of Human Embryonic Stem Cells in Long-Term Hypoxia

    Zachar, Vladimir (et al.)

    Pages 13-24

  • Laboratory-Scale Purification of a Recombinant E-Cadherin-IgG Fc Fusion Protein That Provides a Cell Surface Matrix for Extended Culture and Efficient Subculture of Human Pluripotent Stem Cells

    Nagaoka, Masato (et al.)

    Pages 25-35

  • Scale-Up of Single Cell–Inoculated Suspension Cultures of Human Embryonic Stem Cells

    Singh, Harmeet (et al.)

    Pages 37-50

  • Three-Dimensional Culture for Expansion and Differentiation of Embryonic Stem Cells

    Sun, Guang-wei (et al.)

    Pages 51-58

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-267-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-266-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Embryonic and Induced Pluripotent Stem Cells
Book Subtitle
Lineage-Specific Differentiation Protocols
Editors
  • Kaiming Ye
  • Sha Jin
Series Title
Springer Protocols Handbooks
Copyright
2012
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-267-0
DOI
10.1007/978-1-61779-267-0
Hardcover ISBN
978-1-61779-266-3
Series ISSN
1949-2448
Edition Number
1
Number of Pages
XVI, 486
Number of Illustrations and Tables
40 b/w illustrations, 48 illustrations in colour
Topics