Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 779)
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Table of contents (17 protocols)
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Front Matter
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Overview
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Front Matter
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Kinase Expression, Purification and Activity Assays
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Front Matter
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Studying Kinase Function at the Cellular Level
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Front Matter
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Identification of Kinase Substrates and Phosphorylation-Site Detection
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Front Matter
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Keywords
About this book
Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.
Reviews
From the reviews:
“This book represents a compendium of practical protocols to experimentally address different aspects of plant kinase function and regulation. It is intended to provide a benchtop manual for newcomers to the field but also to present experimental alternatives for the more specialized and advanced user. … this book compiles a wealth of information on how to study plant kinases and provides a useful set of instructions for a wide range of complementary techniques in this field.” (Jörg Kudla, Journal of Plant Physiology, Vol. 169 (15), 2012)Editors and Affiliations
Bibliographic Information
Book Title: Plant Kinases
Book Subtitle: Methods and Protocols
Editors: Nico Dissmeyer, Arp Schnittger
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-264-9
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-263-2Published: 26 August 2011
Softcover ISBN: 978-1-4939-5721-7Published: 23 August 2016
eBook ISBN: 978-1-61779-264-9Published: 26 August 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 312
Topics: Plant Sciences, Proteomics