Methods in Molecular Biology

Plant Kinases

Methods and Protocols

Editors: Dissmeyer, Nico, Schnittger, Arp (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-264-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-263-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes in all organisms. Recent advances in the areas of molecular biology and biochemistry are presenting new possibilities for reaching an unprecedented depth and a proteome-wide understanding of phosphorylation processes in plants as well as in other species. The major goal of Plant Kinases: Methods and Protocols is to provide the experimentalist with a detailed account of the practical steps necessary for successfully carrying out each protocol in his or her own laboratory. Plant protein kinases specifically addressed in this volume are members of the plant MAP kinase cascade, cyclin- and Calcium-dependent protein kinases, and plant sensor and receptor kinases. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Plant Kinases: Methods and Protocols will prove a useful laboratory companion to  both novice and seasoned researchers by facilitating the practical work that will lead them to new and exciting insights in this dynamic field.

Reviews

From the reviews:

“This book represents a compendium of practical protocols to experimentally address different aspects of plant kinase function and regulation. It is intended to provide a benchtop manual for newcomers to the field but also to present experimental alternatives for the more specialized and advanced user. … this book compiles a wealth of information on how to study plant kinases and provides a useful set of instructions for a wide range of complementary techniques in this field.” (Jörg Kudla, Journal of Plant Physiology, Vol. 169 (15), 2012)

Table of contents (17 chapters)

  • Guide to the Book Plant Kinases

    Dissmeyer, Nico (et al.)

    Pages 3-5

  • The Age of Protein Kinases

    Dissmeyer, Nico (et al.)

    Pages 7-52

  • Expression and Purification of Active Protein Kinases from Wheat Germ Extracts

    Sonkoly, Boglárka (et al.)

    Pages 55-63

  • Measurement of Plant Cyclin-Dependent Kinase Activity Using Immunoprecipitation-Coupled and Affinity Purification-Based Kinase Assays and the Baculovirus Expression System

    Harashima, Hirofumi (et al.)

    Pages 65-78

  • Mitogen-Activated Protein Kinase Activity and Reporter Gene Assays in Plants

    Dóczi, Róbert (et al.)

    Pages 79-92

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-264-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-263-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Kinases
Book Subtitle
Methods and Protocols
Editors
  • Nico Dissmeyer
  • Arp Schnittger
Series Title
Methods in Molecular Biology
Series Volume
779
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-264-9
DOI
10.1007/978-1-61779-264-9
Hardcover ISBN
978-1-61779-263-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 312
Topics