Methods in Molecular Biology

Chloroplast Research in Arabidopsis

Methods and Protocols, Volume II

Editors: Jarvis, R Paul (Ed.)

  • Brings together the most important and current techniques in chloroplast research through the unifying lens of the Arabidopsis model system
  • Features subjects like protein aspects, omics studies, and photosynthesis and biochemical analysis
  • Includes expert tips and implementation advice to guarantee ideal experimental results
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eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-237-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-236-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Table of contents (22 chapters)

  • One- and Two-Dimensional Blue Native-PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes

    Kikuchi, Shingo (et al.)

    Pages 3-17

  • Analysis of Thylakoid Protein Complexes by Two-Dimensional Electrophoretic Systems

    Sirpiö, Sari (et al.)

    Pages 19-30

  • Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification

    Andrès, Charles (et al.)

    Pages 31-49

  • Studying Interactions Between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation and Förster Resonance Energy Transfer

    Maple, Jodi (et al.)

    Pages 51-65

  • Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods

    Tripp, Joanna (et al.)

    Pages 67-90

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-237-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-236-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chloroplast Research in Arabidopsis
Book Subtitle
Methods and Protocols, Volume II
Editors
  • R Paul Jarvis
Series Title
Methods in Molecular Biology
Series Volume
775
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-237-3
DOI
10.1007/978-1-61779-237-3
Hardcover ISBN
978-1-61779-236-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 432
Number of Illustrations and Tables
56 b/w illustrations, 5 illustrations in colour
Topics