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  • © 2011

Chloroplast Research in Arabidopsis

Methods and Protocols, Volume I

Editors:

  • Brings together the most important and current techniques in chloroplast research through the unifying lens of the Arabidopsis model system
  • Features subjects like genetics, cytology, and protein studies
  • Includes expert tips and implementation advice to guarantee ideal experimental results

Part of the book series: Methods in Molecular Biology (MIMB, volume 774)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-x
  2. Gene Expression and Protein Accumulation

    1. Front Matter

      Pages 149-149
    2. Isolation, Quantification, and Analysis of Chloroplast DNA

      • Beth A. Rowan, Arnold J. Bendich
      Pages 151-170
    3. Measurement of Transcription Rates in Arabidopsis Chloroplasts

      • Yan O. Zubo, Thomas Börner, Karsten Liere
      Pages 171-182
    4. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts

      • Lars L. E. Sjögren, Adrian K. Clarke
      Pages 225-240

About this book

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Editors and Affiliations

  • , Department of Biology, University of Leicester, Leicester, United Kingdom

    R. Paul Jarvis

Bibliographic Information

  • Book Title: Chloroplast Research in Arabidopsis

  • Book Subtitle: Methods and Protocols, Volume I

  • Editors: R. Paul Jarvis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-234-2

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-233-5Published: 09 August 2011

  • Softcover ISBN: 978-1-4939-5850-4Published: 23 August 2016

  • eBook ISBN: 978-1-61779-234-2Published: 09 August 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 374

  • Topics: Plant Sciences, Plant Biochemistry

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access