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Molecular Methods for Evolutionary Genetics

  • Book
  • © 2011

Overview

Editors:
  1. Virginie Orgogozo

    1. CNRS UMR7592, Université Paris VII, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Matthew V. Rockman

    1. Dept. Biology, New York University, New York, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 772)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvi
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  2. Characterizing the Genome

    1. Front Matter

      Pages 1-1
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    2. Genome Size Determination Using Flow Cytometry of Propidium Iodide-Stained Nuclei

      • Emily E. Hare, J. Spencer Johnston
      Pages 3-12

    3. Chromosome Analysis in Invertebrates and Vertebrates

      • David M. Rowell, Shu Ly Lim, Frank Grutzner
      Pages 13-35

    4. Genomic Libraries: I. Construction and Screening of Fosmid Genomic Libraries

      • Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, Simon W. Baxter
      Pages 37-58

    5. Genomic Libraries: II. Subcloning, Sequencing, and Assembling Large-Insert Genomic DNA Clones

      • Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, Simon W. Baxter
      Pages 59-81

  3. Targeting Regions of the Genome

    1. Front Matter

      Pages 83-83
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    2. Reduced Representation Methods for Subgenomic Enrichment and Next-Generation Sequencing

      • Jeffrey M. Good
      Pages 85-103

    3. Accessing the Transcriptome: How to Normalize mRNA Pools

      • Heiko Vogel, Christopher W. Wheat
      Pages 105-128

    4. Transcriptome Sequencing Goals, Assembly, and Assessment

      • Christopher W. Wheat, Heiko Vogel
      Pages 129-144

    5. Rapid Retrieval of DNA Target Sequences by Primer Extension Capture

      • Adrian W. Briggs
      Pages 145-154

  4. Measuring Genetic Diversity

    1. Front Matter

      Pages 155-155
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    2. SNP Discovery and Genotyping for Evolutionary Genetics Using RAD Sequencing

      • Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric A. Johnson, William A. Cresko
      Pages 157-178

    3. DNA Microarray-Based Mutation Discovery and Genotyping

      • David Gresham
      Pages 179-191

    4. Genotyping with Sequenom

      • Martina Bradić, João Costa, Ivo M. Chelo
      Pages 193-210

    5. Isolating Microsatellite Loci: Looking Back, Looking Ahead

      • José A. Andrés, Steven M. Bogdanowicz
      Pages 211-232

    6. Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids Using Array Oligo Selector

      • Amy A. Caudy
      Pages 233-241

  5. Obtaining Candidate Gene Sequences

    1. Front Matter

      Pages 243-243
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    2. Identification of Homologous Gene Sequences by PCR with Degenerate Primers

      • Michael Lang, Virginie Orgogozo
      Pages 245-256

    3. Characterizing cDNA Ends by Circular RACE

      • Patrick T. McGrath
      Pages 257-265
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Keywords

About this book

We are entering a particularly fruitful period in evolutionary genetics, as rapid technological progress transforms the investigation of genetic variation within and between species. Molecular Methods for Evolutionary Genetics is a collection of advanced molecular biology protocols and general overviews intended to represent the essential methods currently bringing evolutionary genetics to fruition. Divided into six thematic sections, this volume covers methods for characterizing genomes, diverse approaches to enrich DNA for subsets of the genome prior to sequencing, and state-of-the-art protocols for sampling genetic variation for genetic mapping studies and population genetic studies (RAD sequencing, Sequenom, microarrays, etc.). The volume concludes by focusing on methods to study candidate genes, from obtaining their sequences and analyzing their transcripts to experimentally manipulating their activities in vivo. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Molecular Methods for Evolutionary Genetics serves as a rich resource to biologists interested in evolution, whether they be specialists or beginners in molecular biology.

Editors and Affiliations

  • CNRS UMR7592, Université Paris VII, Paris, France

    Virginie Orgogozo

  • Dept. Biology, New York University, New York, USA

    Matthew V. Rockman

Bibliographic Information

  • Book Title: Molecular Methods for Evolutionary Genetics

  • Editors: Virginie Orgogozo, Matthew V. Rockman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-228-1

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-227-4Published: 29 September 2011

  • Softcover ISBN: 978-1-4939-5861-0Published: 23 August 2016

  • eBook ISBN: 978-1-61779-228-1Published: 09 November 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 503

  • Number of Illustrations: 77 b/w illustrations, 4 illustrations in colour

  • Topics: Human Genetics, Gene Function

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