Methods in Molecular Biology

Human Pluripotent Stem Cells

Methods and Protocols

Editors: Schwartz, Philip H., Wesselschmidt, Robin L. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $139.00
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  • ISBN 978-1-61779-201-4
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Softcover $179.00
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  • ISBN 978-1-4939-5802-3
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About this book

Almost daily, new technologies are being presented that move the field of human pluripotent stem cell research towards a future that may yield highly-effective, personalized medical treatments.  Three enabling technologies at hand for human PSCs are 1) directed reprogramming of somatic cells, which eliminate many of the ethical issues associated with the derivation and use of human PSCs, increase genetic diversity of the available human PSC lines, and give rise to better in vitro human disease models; 2) the discovery that a Rho-associated protein Kinase (ROCK) inhibitor allows for efficient single cell passaging and cryopreservation, increasing the efficiency and reliability of hPSC culture; and 3) defined, animal-component-free media, which lay the groundwork for simplified scale-up for therapeutic applications, differentiation protocols, and toxicology screens. The aforementioned technologies can be found in Human Pluripotent Stem Cells: Methods and Protocols, a compilation of 33 detailed protocols in six categories of PSC research that cover laboratory essentials and the derivation of new PSC lines, including induced PSC lines, as well as their growth, maintenance, characterization, genetic manipulation, and differentiation. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

            Authoritative and accessible, Human Pluripotent Stem Cells: Methods and Protocols serves as an ideal guide to scientists conducting their own pluripotent cell research programs and makes great strides towards furthering human knowledge and, ultimately, improving the human condition.

Reviews

From the reviews:

“The volume is a compilation of 33 detailed protocols divided into six parts … . All the protocols are superbly illustrated with many figures and charts presented in full colours. I think this book is a must for all those are working with human pluripotent stem cells, no matter if students entering the field or colleagues already involved, each of them will get the chance to sharpen their conceptual insights.” (Carlo Alberto Redi, European Journal of Histochemistry, Vol. 56, 2012)


Table of contents (33 chapters)

  • The Stem Cell Laboratory: Design, Equipment, and Oversight

    Wesselschmidt, Robin L. (et al.)

    Pages 3-13

  • Stem Cell Banks: Preserving Cell Lines, Maintaining Genetic Integrity, and Advancing Research

    Healy, Lyn (et al.)

    Pages 15-27

  • Embryonic Stem Cell Derivation from Human Embryos

    Lerou, Paul

    Pages 31-35

  • Derivation of Human Parthenogenetic Stem Cell Lines

    Turovets, Nikolay (et al.)

    Pages 37-54

  • Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfer

    Miller, Justine D. (et al.)

    Pages 55-65

Buy this book

eBook $139.00
price for USA (gross)
  • ISBN 978-1-61779-201-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA
  • ISBN 978-1-61779-200-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA
  • ISBN 978-1-4939-5802-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Human Pluripotent Stem Cells
Book Subtitle
Methods and Protocols
Editors
  • Philip H. Schwartz
  • Robin L. Wesselschmidt
Series Title
Methods in Molecular Biology
Series Volume
767
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-201-4
DOI
10.1007/978-1-61779-201-4
Hardcover ISBN
978-1-61779-200-7
Softcover ISBN
978-1-4939-5802-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XX, 468
Topics