Methods in Molecular Biology

In Vitro Neurotoxicology

Methods and Protocols

Editors: Costa, Lucio G., Giordano, Gennaro, Guizzetti, Marina (Eds.)

  • Serves a wide audience seeking to assess or characterize potential neurotoxicity
  • Includes detailed, lab-ready protocols for numerous model system, both mammalian and non-mammalian
  • Features key tips and expert implementation advice for ideal results
see more benefits

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-170-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-169-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5858-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems.  In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems.  Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.

Reviews

From the book reviews:

“The variety of fresh and effective laboratory techniques makes this attractive book a necessary companion for researchers, teachers and students in this field. The editors ought to be commended for bringing together into a single, easy-access volume information on so abstruse a topic as in vitro neurotoxicology. The lay-out of the book as well as the clear and high resolution diagrams and plates are some of the most attractive attributes … .” (Jolocam Mbabazi, International Journal of Environmental Studies, Vol. 69 (6), 2012)

“This volume … is a concise review of cellular, biochemical, and molecular methodological protocols for diverse areas of in vitro neurotoxicity studies. … This collection will be useful to those who are interested in studying the neurotoxicity of environmental contaminants, drugs, or other chemicals. Specifically, it will be of value to graduate students, postdoctoral fellows, and faculty in academia. … The book also provides useful methodological guidelines and examples that will be helpful to researchers who are relatively new to this field.” (Bao Ting Zhu, Doody’s Book Reviews, December, 2011)

Table of contents (28 chapters)

  • In Vitro Neurotoxicology: An Introduction

    Costa, Lucio G. (et al.)

    Pages 1-9

  • Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies

    Giordano, Gennaro (et al.)

    Pages 13-27

  • Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures

    Tiffany-Castiglioni, Evelyn (et al.)

    Pages 29-48

  • Microglia Cell Culture: A Primer for the Novice

    Witting, Anke (et al.)

    Pages 49-66

  • Neural Stem Cells for Developmental Neurotoxicity Studies

    Tofighi, Roshan (et al.)

    Pages 67-80

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-170-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-169-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $169.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-5858-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
In Vitro Neurotoxicology
Book Subtitle
Methods and Protocols
Editors
  • Lucio G. Costa
  • Gennaro Giordano
  • Marina Guizzetti
Series Title
Methods in Molecular Biology
Series Volume
758
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-170-3
DOI
10.1007/978-1-61779-170-3
Hardcover ISBN
978-1-61779-169-7
Softcover ISBN
978-1-4939-5858-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 415
Number of Illustrations and Tables
66 b/w illustrations
Topics