Methods in Molecular Biology

Laser Capture Microdissection

Methods and Protocols

Editors: Murray, Graeme I. (Ed.)

  • Provides practical methods and protocols involving tissue-based laser microdissection, ideal for labs around the globe
  • Fully updates and reexamines the popular first edition
  • Features key notes and expert implementation advice
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-163-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-162-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6080-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics.  In second edition of Laser Capture Microdissection: Methods and Protocols, experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies.  The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems.  The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.

Table of contents (40 chapters)

  • Laser Capture Microdissection: Methods and Applications

    DeCarlo, Kristen (et al.)

    Pages 1-15

  • Laser Microdissection for Gene Expression Profiling

    Field, Lori A. (et al.)

    Pages 17-45

  • Gene Expression Using the PALM System

    Lu, Jian-Xin (et al.)

    Pages 47-56

  • Immunoguided Microdissection Techniques

    Tangrea, Michael A. (et al.)

    Pages 57-66

  • Optimized RNA Extraction from Non-deparaffinized, Laser-Microdissected Material

    Jonigk, Danny (et al.)

    Pages 67-75

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-163-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-61779-162-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA
valid through November 5, 2017
  • ISBN 978-1-4939-6080-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Laser Capture Microdissection
Book Subtitle
Methods and Protocols
Editors
  • Graeme I. Murray
Series Title
Methods in Molecular Biology
Series Volume
755
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-163-5
DOI
10.1007/978-1-61779-163-5
Hardcover ISBN
978-1-61779-162-8
Softcover ISBN
978-1-4939-6080-4
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVII, 493
Number of Illustrations and Tables
96 b/w illustrations, 1 illustrations in colour
Topics