Methods in Molecular Biology

Plant Transcription Factors

Methods and Protocols

Editors: Yuan, Ling, Perry, Sharyn E. (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $129.00
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  • ISBN 978-1-61779-154-3
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Hardcover $169.00
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  • ISBN 978-1-61779-153-6
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About this book

Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors. 

Reviews

From the reviews:

“It represents a collection of protocols for performing analysis on plant transcription factors (TFs). … this book will be very useful to many research groups. The different protocols are described in a user-friendly manner which makes it an excellent source of information for students and scientists that are new to the field. In addition, it is well suited to serve as a basis to optimize the broad array of techniques relevant to the field of plant transcription factor research.” (Tzvetina Brumbarova, Journal of Plant Physiology, Vol. 169 (13), 2012)


Table of contents (19 chapters)

  • MADS and More: Transcription Factors That Shape the Plant

    Melzer, Rainer (et al.)

    Pages 3-18

  • In Silico Mining and PCR-Based Approaches to Transcription Factor Discovery in Non-model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers

    Liu, Jun-Jun (et al.)

    Pages 21-43

  • Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System

    Pyvovarenko, Tatiana (et al.)

    Pages 45-66

  • A Transposon-Based Activation Tagging System for Gene Function Discovery in Arabidopsis

    Marsch-Martínez, Nayelli

    Pages 67-83

  • CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors

    Mitsuda, Nobutaka (et al.)

    Pages 87-105

Buy this book

eBook $129.00
price for USA (gross)
  • ISBN 978-1-61779-154-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.00
price for USA
  • ISBN 978-1-61779-153-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Plant Transcription Factors
Book Subtitle
Methods and Protocols
Editors
  • Ling Yuan
  • Sharyn E. Perry
Series Title
Methods in Molecular Biology
Series Volume
754
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-154-3
DOI
10.1007/978-1-61779-154-3
Hardcover ISBN
978-1-61779-153-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 347
Topics