Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 754)
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Table of contents (19 protocols)
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Front Matter
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Introduction
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Front Matter
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Identification of Transcription Factors
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Front Matter
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Protein–Protein Interaction and Other Means for Activity Control
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Front Matter
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Protein-Protein Interaction and Other Means for Activity Control
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Protein–DNA Interaction
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Front Matter
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Keywords
About this book
Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.
Reviews
From the reviews:
“It represents a collection of protocols for performing analysis on plant transcription factors (TFs). … this book will be very useful to many research groups. The different protocols are described in a user-friendly manner which makes it an excellent source of information for students and scientists that are new to the field. In addition, it is well suited to serve as a basis to optimize the broad array of techniques relevant to the field of plant transcription factor research.” (Tzvetina Brumbarova, Journal of Plant Physiology, Vol. 169 (13), 2012)
Editors and Affiliations
Bibliographic Information
Book Title: Plant Transcription Factors
Book Subtitle: Methods and Protocols
Editors: Ling Yuan, Sharyn E. Perry
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-154-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-153-6Published: 08 July 2011
Softcover ISBN: 978-1-4939-5844-3Published: 23 August 2016
eBook ISBN: 978-1-61779-154-3Published: 08 July 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 347