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Methods in Molecular Biology

Bioconjugation Protocols

Strategies and Methods

Editors: Mark, Sonny S. (Ed.)

  • Readily reproducible techniques for synthesizing functional biomolecular reagents
  • Emphasis on the interplay of advanced organic synthesis, molecular biology, surface biotechnology and biomaterials engineering
  • Step-by-step laboratory protocols for the preparation of protein, nucleic acid, glycosyl and lipid conjugates
  • Applications ranging from novel biomedical diagnostics to therapeutics and biomaterials
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eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-151-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-150-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Contemporary approaches to the synthesis of chemically modified biomacromolecules (proteins, nucleic acids, lipids, and carbohydrates) not only require efficient means to control conjugation and the specific site of attachment of the conjugated moiety but also the effective use of recent developments in the fields of pharmaceutical chemistry, biomolecular/polymer engineering, and nanobiotechnology.  In this second edition of Bioconjugation Protocols:  Strategies and Methods, expert researchers update the classic methods and introduce valuable new approaches that go beyond basic conjugation techniques to include elements from advanced organic synthesis, molecular biology, surface biotechnology, materials science, and nanobioscience/engineering.  These readily reproducible methods cover the preparation of biomolecular conjugates using a variety of labeling techniques and semisynthetic approaches.  Additional chapters address the biofunctionalization of surface structures, including organic/inorganic thin films, as well as various types of nanostructures (magnetic nanoparticles, quantum dots, carbon nanotubes, and silicon nanowire devices).  All the protocols follow the successful Methods in Molecular BiologyTM series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and highly practical, Bioconjugation Protocols: Strategies and Methods, Second Edition offers both novice and experienced researchers access to the broad array of techniques needed to carry out the semisynthesis of functional biomolecular reagents and/or the biofunctionalization of surfaces and structures of unique interest for a wide variety of applications, ranging from novel biomedical diagnostics to powerful new therapeutics to advanced biomaterials.

Table of contents (36 chapters)

  • Site-Specific Labeling of Proteins for Single-Molecule FRET Measurements Using Genetically Encoded Ketone Functionalities

    Lemke, Edward A.

    Pages 3-15

  • Enzymatically Catalyzed Conjugation of a Biodegradable Polymer to Proteins and Small Molecules Using Microbial Transglutaminase

    Besheer, Ahmed (et al.)

    Pages 17-27

  • Synthesis of Drug/Dye-Incorporated Polymer–Protein Hybrids

    Dolai, Sukanta (et al.)

    Pages 29-42

  • Dye/DNA Conjugates as Multiple Labels for Antibodies in Sensitive Fluorescence Immunoassays

    Zhang, Qin (et al.)

    Pages 43-54

  • Chemoselective Modification of Viral Proteins Bearing Metabolically Introduced “Clickable” Amino Acids and Sugars

    Banerjee, Partha S. (et al.)

    Pages 55-66

Buy this book

eBook $149.00
price for USA (gross)
  • ISBN 978-1-61779-151-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $189.00
price for USA
  • ISBN 978-1-61779-150-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bioconjugation Protocols
Book Subtitle
Strategies and Methods
Editors
  • Sonny S. Mark
Series Title
Methods in Molecular Biology
Series Volume
751
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-151-2
DOI
10.1007/978-1-61779-151-2
Hardcover ISBN
978-1-61779-150-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XVI, 605
Number of Illustrations and Tables
182 b/w illustrations
Topics