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RNAi and Plant Gene Function Analysis

Methods and Protocols

  • Book
  • © 2011

Overview

Editors:
  1. Hiroaki Kodama

    1. Graduate School of Horticulture, Chiba University, Chiba, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Atsushi Komamine

    1. Evolutionary Biology, Research Institute of, Tokyo, Setagaya, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 744)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
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  2. Overview of Plant RNAi

    • Yuichiro Watanabe
    Pages 1-11

  3. Caveat of RNAi in Plants: The Off-Target Effect

    • Muthappa Senthil-Kumar, Kirankumar S. Mysore
    Pages 13-25

  4. Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies

    • Toshiya Muranaka
    Pages 27-35

  5. Heat-Inducible RNAi for Gene Functional Analysis in Plants

    • Frédéric Masclaux, Jean-Philippe Galaud
    Pages 37-55

  6. Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens

    • Basel Khraiwesh, Isam Fattash, M. Asif Arif, Wolfgang Frank
    Pages 57-79

  7. Virus-Induced Gene Silencing in Ornamental Plants

    • Cai-Zhong Jiang, Jen-Chih Chen, Michael Reid
    Pages 81-96

  8. Local RNA Silencing Mediated by Agroinfiltration

    • Jutta Maria Helm, Elena Dadami, Kriton Kalantidis
    Pages 97-108

  9. Direct Transfer of Synthetic Double-Stranded RNA into Protoplasts of Arabidopsis thaliana

    • Ha-il Jung, Zhiyang Zhai, Olena K. Vatamaniuk
    Pages 109-127

  10. Detection of Long and Short Double-Stranded RNAs

    • Toshiyuki Fukuhara, Syunichi Urayama, Ryo Okada, Eri Kiyota, Hiromitsu Moriyama
    Pages 129-144

  11. Quantitative Stem-Loop RT-PCR for Detection of MicroRNAs

    • Erika Varkonyi-Gasic, Roger P. Hellens
    Pages 145-157

  12. Large-Scale Sequencing of Plant Small RNAs

    • William P. Donovan, Yuanji Zhang, Miya D. Howell
    Pages 159-173

  13. Computational Prediction of Plant miRNA Targets

    • Ying-Hsuan Sun, Shanfa Lu, Rui Shi, Vincent L. Chiang
    Pages 175-186

  14. Bisulfite Sequencing for Cytosine-Methylation Analysis in Plants

    • Nazmul Haque, Masamichi Nishiguchi
    Pages 187-197

  15. Using Nuclear Run-On Transcription Assays in RNAi Studies

    • Basel Khraiwesh
    Pages 199-209

  16. Proteomic Analysis of RNA Interference Induced Knockdown Plant

    • Sang Yeol Lee, Kyun Oh Lee
    Pages 211-224

  17. Comparative Analysis of Phosphoprotein Expression Using 2D-DIGE

    • Tomoya Asano, Takumi Nishiuchi
    Pages 225-233

  18. Back Matter

    Pages 235-244
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Keywords

About this book

The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.

Editors and Affiliations

  • Graduate School of Horticulture, Chiba University, Chiba, Japan

    Hiroaki Kodama

  • Evolutionary Biology, Research Institute of, Tokyo, Setagaya, Japan

    Atsushi Komamine

Bibliographic Information

  • Book Title: RNAi and Plant Gene Function Analysis

  • Book Subtitle: Methods and Protocols

  • Editors: Hiroaki Kodama, Atsushi Komamine

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-61779-123-9

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2011

  • Hardcover ISBN: 978-1-61779-122-2Published: 04 May 2011

  • Softcover ISBN: 978-1-62703-932-1Published: 29 November 2014

  • eBook ISBN: 978-1-61779-123-9Published: 30 April 2011

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 244

  • Topics: Plant Genetics and Genomics

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