Methods in Molecular Biology

RNAi and Plant Gene Function Analysis

Methods and Protocols

Editors: Kodama, Hiroaki, Komamine, Atsushi (Eds.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-123-9
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  • Immediate eBook download after purchase
Hardcover $139.00
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  • ISBN 978-1-61779-122-2
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  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
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  • ISBN 978-1-62703-932-1
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About this book

The use of RNAi technology is essential for most plant science researchers. As DNA sequence information increases, so the need for functional annotation of target genes also increases. Authoritative and accessible, RNAi and Plant Gene Function Analysis: Methods and Protocols seeks to serve as an ideal guide to scientists of all backgrounds looking to further their knowledge of this fundamental science. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

In addition to the functional annotation of unknown genes, RNAi technology has been applied to the genetic engineering of important plant metabolites including starches, oils, and storage proteins. RNAi has been used to engineer plants resistant to plant viruses and also to nematodes and insects. RNAi and Plant Gene Function Analysis: Methods and Protocols will provide both professionals and novices of plant biotechnology and breeding research with essential tips for designing experiments meant to explore plant gene function.

Table of contents (16 chapters)

  • Overview of Plant RNAi

    Watanabe, Yuichiro

    Pages 1-11

  • Caveat of RNAi in Plants: The Off-Target Effect

    Senthil-Kumar, Muthappa (et al.)

    Pages 13-25

  • Plant Gateway Vectors for RNAi as a Tool for Functional Genomic Studies

    Muranaka, Toshiya

    Pages 27-35

  • Heat-Inducible RNAi for Gene Functional Analysis in Plants

    Masclaux, Frédéric (et al.)

    Pages 37-55

  • Gene Function Analysis by Artificial MicroRNAs in Physcomitrella patens

    Khraiwesh, Basel (et al.)

    Pages 57-79

Buy this book

eBook $109.00
price for USA (gross)
  • ISBN 978-1-61779-123-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $139.00
price for USA
  • ISBN 978-1-61779-122-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $139.00
price for USA
  • ISBN 978-1-62703-932-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNAi and Plant Gene Function Analysis
Book Subtitle
Methods and Protocols
Editors
  • Hiroaki Kodama
  • Atsushi Komamine
Series Title
Methods in Molecular Biology
Series Volume
744
Copyright
2011
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC
eBook ISBN
978-1-61779-123-9
DOI
10.1007/978-1-61779-123-9
Hardcover ISBN
978-1-61779-122-2
Softcover ISBN
978-1-62703-932-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 244
Topics